+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 2n5e | ||||||
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タイトル | The 3D solution structure of discoidal high-density lipoprotein particles | ||||||
要素 | Apolipoprotein A-IApolipoprotein AI | ||||||
キーワード | LIPID BINDING PROTEIN / nanodisc / HDL / lipoproteins (リポタンパク質) / cardiovascular disease (心血管疾患) | ||||||
機能・相同性 | 機能・相同性情報 Defective ABCA1 causes TGD / Scavenging by Class B Receptors / HDL clearance / high-density lipoprotein particle receptor binding / spherical high-density lipoprotein particle / positive regulation of hydrolase activity / negative regulation of response to cytokine stimulus / regulation of intestinal cholesterol absorption / protein oxidation / vitamin transport ...Defective ABCA1 causes TGD / Scavenging by Class B Receptors / HDL clearance / high-density lipoprotein particle receptor binding / spherical high-density lipoprotein particle / positive regulation of hydrolase activity / negative regulation of response to cytokine stimulus / regulation of intestinal cholesterol absorption / protein oxidation / vitamin transport / cholesterol import / high-density lipoprotein particle binding / ABC transporters in lipid homeostasis / blood vessel endothelial cell migration / negative regulation of heterotypic cell-cell adhesion / apolipoprotein receptor binding / apolipoprotein A-I receptor binding / negative regulation of cytokine production involved in immune response / negative regulation of cell adhesion molecule production / HDL assembly / peptidyl-methionine modification / negative regulation of very-low-density lipoprotein particle remodeling / phosphatidylcholine biosynthetic process / glucocorticoid metabolic process / phosphatidylcholine metabolic process / Chylomicron remodeling / phosphatidylcholine-sterol O-acyltransferase activator activity / positive regulation of phospholipid efflux / lipid storage / Chylomicron assembly / positive regulation of cholesterol metabolic process / phospholipid homeostasis / high-density lipoprotein particle clearance / high-density lipoprotein particle remodeling / phospholipid efflux / chemorepellent activity / cholesterol transfer activity / reverse cholesterol transport / high-density lipoprotein particle assembly / very-low-density lipoprotein particle / cholesterol transport / positive regulation of CoA-transferase activity / lipoprotein biosynthetic process / high-density lipoprotein particle / regulation of Cdc42 protein signal transduction / triglyceride homeostasis / HDL remodeling / endothelial cell proliferation / negative regulation of interleukin-1 beta production / Scavenging by Class A Receptors / cholesterol efflux / cholesterol binding / negative chemotaxis / positive regulation of Rho protein signal transduction / adrenal gland development / cholesterol biosynthetic process / endocytic vesicle / negative regulation of tumor necrosis factor-mediated signaling pathway / positive regulation of cholesterol efflux / Scavenging of heme from plasma / positive regulation of phagocytosis / positive regulation of substrate adhesion-dependent cell spreading / Retinoid metabolism and transport / positive regulation of stress fiber assembly / endocytic vesicle lumen / heat shock protein binding / cholesterol metabolic process / cholesterol homeostasis / integrin-mediated signaling pathway / Post-translational protein phosphorylation / regulation of protein phosphorylation / Heme signaling / phospholipid binding / PPARA activates gene expression / negative regulation of inflammatory response / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / extracellular vesicle / Platelet degranulation / amyloid-beta binding / cytoplasmic vesicle / secretory granule lumen / collagen-containing extracellular matrix / blood microparticle / エンドソーム / protein stabilization / G protein-coupled receptor signaling pathway / Amyloid fiber formation / 小胞体 / signaling receptor binding / enzyme binding / protein homodimerization activity / extracellular space / extracellular exosome / extracellular region / identical protein binding / 細胞膜 / 細胞質基質 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | 溶液NMR / simulated annealing | ||||||
Model details | lowest energy, model1 | ||||||
データ登録者 | Bibow, S. / Polyhach, Y. / Eichmann, C. / Chi, C.N. / Kowal, J. / Stahlberg, H. / Jeschke, G. / Guentert, P. / Riek, R. | ||||||
引用 | ジャーナル: Nat.Struct.Mol.Biol. / 年: 2017 タイトル: Solution structure of discoidal high-density lipoprotein particles with a shortened apolipoprotein A-I. 著者: Bibow, S. / Polyhach, Y. / Eichmann, C. / Chi, C.N. / Kowal, J. / Albiez, S. / McLeod, R.A. / Stahlberg, H. / Jeschke, G. / Guntert, P. / Riek, R. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 2n5e.cif.gz | 1 MB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb2n5e.ent.gz | 906.4 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 2n5e.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/n5/2n5e ftp://data.pdbj.org/pub/pdb/validation_reports/n5/2n5e | HTTPS FTP |
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-関連構造データ
類似構造データ | |
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その他のデータベース |
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 19461.973 Da / 分子数: 2 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: APOA1 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P02647 |
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-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||||||
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NMR実験 |
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-試料調製
詳細 | 内容: 0.5-1.0 mM [U-99% 13C; U-99% 15N] H2O, 1 mM stereospecific Methyl-labeling H2O, 1 mM selective unlabeling H2O, 90% H2O/10% D2O 溶媒系: 90% H2O/10% D2O | ||||||||||||||||||||||||
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試料 |
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試料状態 | イオン強度: 100 / pH: 7.4 / 圧: ambient / 温度: 316 K |
-NMR測定
NMRスペクトロメーター |
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-解析
NMR software |
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精密化 | 手法: simulated annealing / ソフトェア番号: 1 | ||||||||||||||||
代表構造 | 選択基準: lowest energy | ||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: target function / 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 10 |