+Open data
-Basic information
Entry | Database: PDB / ID: 2n0f | ||||||
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Title | NMR structure of Neuromedin C in 60% TFE | ||||||
Components | Neuromedin C (NMC) | ||||||
Keywords | HORMONE / Neuropeptide / Feeding regulation | ||||||
Function / homology | Function and homology information negative regulation of voltage-gated sodium channel activity / cellular response to sodium phosphate / response to external biotic stimulus / positive regulation of respiratory gaseous exchange / negative regulation of voltage-gated potassium channel activity / positive regulation of behavioral fear response / psychomotor behavior / neuropeptide hormone activity / positive regulation of vascular associated smooth muscle cell apoptotic process / mast cell degranulation ...negative regulation of voltage-gated sodium channel activity / cellular response to sodium phosphate / response to external biotic stimulus / positive regulation of respiratory gaseous exchange / negative regulation of voltage-gated potassium channel activity / positive regulation of behavioral fear response / psychomotor behavior / neuropeptide hormone activity / positive regulation of vascular associated smooth muscle cell apoptotic process / mast cell degranulation / positive regulation of phospholipase C-activating G protein-coupled receptor signaling pathway / neuronal dense core vesicle / positive regulation of peptide hormone secretion / social behavior / neuropeptide signaling pathway / Peptide ligand-binding receptors / ossification / Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1) / G alpha (q) signalling events / regulation of gene expression / secretory granule lumen / neuron projection / signaling receptor binding / signal transduction / extracellular space / extracellular region Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / distance geometry | ||||||
Authors | Adrover, M. / Sanchis, P. / Vilanova, B. / Pauwels, K. / Martorell, G. / Perez, J. | ||||||
Citation | Journal: RSC ADV / Year: 2015 Title: Conformational ensembles of neuromedin C reveal a progressive coil-helix transition within a binding-induced folding mechanism. Authors: Adrover, M. / Sanchis, P. / Vilanova, B. / Pauwels, K. / Martorell, G. / Perez, J.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2n0f.cif.gz | 49.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2n0f.ent.gz | 33.9 KB | Display | PDB format |
PDBx/mmJSON format | 2n0f.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/n0/2n0f ftp://data.pdbj.org/pub/pdb/validation_reports/n0/2n0f | HTTPS FTP |
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-Related structure data
Related structure data | 2n0bC 2n0cC 2n0dC 2n0eC 2n0gC 2n0hC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 1121.295 Da / Num. of mol.: 1 / Fragment: UNP residues 41-50 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) / References: UniProt: P07492 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 5.0 mM Neuromedin C, 10 mM sodium acetate, 1.6 mM DSS, 60% d-TFE, 10% D2O, 30% H2O Solvent system: 60% d-TFE/10% D2O/30% H2O | ||||||||||||
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Sample |
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Sample conditions | Ionic strength: 0.01 / pH: 4.0 / Pressure: ambient / Temperature: 288 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: distance geometry / Software ordinal: 1 | |||||||||||||||||||||
NMR constraints | NOE constraints total: 107 / NOE intraresidue total count: 80 / NOE long range total count: 0 / NOE medium range total count: 3 / NOE sequential total count: 24 | |||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 20 / Conformers submitted total number: 19 |