+Open data
-Basic information
Entry | Database: PDB / ID: 2mxt | ||||||
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Title | NMR structure of the acidic domain of SYNCRIP (hnRNPQ) | ||||||
Components | Heterogeneous nuclear ribonucleoprotein Q | ||||||
Keywords | SPLICING / Structural Genomics / PSI-Biology / Acidic domain / SYNCRIP / Splicing factor / Joint Center for Structural Genomics / JCSG / Partnership for T-Cell Biology / TCELL | ||||||
Function / homology | Function and homology information mRNA editing complex / CRD-mediated mRNA stability complex / mRNA modification / negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / mCRD-mediated mRNA stability complex / : / negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / CRD-mediated mRNA stabilization / histone pre-mRNA 3'end processing complex / positive regulation of cytoplasmic translation ...mRNA editing complex / CRD-mediated mRNA stability complex / mRNA modification / negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / mCRD-mediated mRNA stability complex / : / negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / CRD-mediated mRNA stabilization / histone pre-mRNA 3'end processing complex / positive regulation of cytoplasmic translation / GAIT complex / RNA processing / catalytic step 2 spliceosome / RNA splicing / mRNA 5'-UTR binding / mRNA splicing, via spliceosome / osteoblast differentiation / cellular response to type II interferon / negative regulation of translation / ribonucleoprotein complex / endoplasmic reticulum / RNA binding / nucleoplasm / membrane / nucleus / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / molecular dynamics | ||||||
Authors | Serrano, P. / Wuthrich, K. / Beuck, C. / Joint Center for Structural Genomics (JCSG) / Partnership for T-Cell Biology (TCELL) | ||||||
Citation | Journal: To be Published Title: NMR structure of the acidic domain of SYNCRIP Authors: Serrano, P. / Beuck, C. / Wuthrich, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2mxt.cif.gz | 558.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2mxt.ent.gz | 476.1 KB | Display | PDB format |
PDBx/mmJSON format | 2mxt.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mx/2mxt ftp://data.pdbj.org/pub/pdb/validation_reports/mx/2mxt | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 9494.643 Da / Num. of mol.: 1 / Fragment: acidic domain (UNP residues 24-107) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: SYNCRIP, HNRPQ, NSAP1 / Plasmid: pET28b / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: O60506 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.2 mM [U-99% 13C; U-98% 15N] protein, 20 mM sodium phosphate, 50 mM sodium chloride, 5 mM sodium azide, 95% H2O/5% D2O Solvent system: 95% H2O/5% D2O | ||||||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 0.0798 / pH: 6.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software | Name: OPALp / Classification: refinement |
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Refinement | Method: molecular dynamics / Software ordinal: 1 |
NMR constraints | NOE constraints total: 1516 / NOE intraresidue total count: 401 / NOE long range total count: 274 / NOE medium range total count: 471 / NOE sequential total count: 370 |
NMR representative | Selection criteria: lowest energy |
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 40 / Conformers submitted total number: 19 |