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Yorodumi- PDB-2mvf: Structural insight into an essential assembly factor network on t... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2mvf | ||||||
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Title | Structural insight into an essential assembly factor network on the pre-ribosome | ||||||
Components | Uncharacterized protein | ||||||
Keywords | RIBOSOMAL PROTEIN / RIBOSOME BIOGENESIS | ||||||
Function / homology | Ribosomal biogenesis NSA2 family / Ribosomal protein S8e/ribosomal biogenesis NSA2 / Ribosomal protein S8e / rRNA processing / ribonucleoprotein complex / nucleolus / Ribosome biogenesis protein NSA2 homolog Function and homology information | ||||||
Biological species | Chaetomium thermophilum (fungus) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model1 | ||||||
Authors | Lee, W. / Bassler, J. / Paternoga, H. / Holdermann, I. / Thomas, M. / Granneman, S. / Barrio-Garcia, C. / Nyarko, A. / Stier, G. / Clark, S.A. ...Lee, W. / Bassler, J. / Paternoga, H. / Holdermann, I. / Thomas, M. / Granneman, S. / Barrio-Garcia, C. / Nyarko, A. / Stier, G. / Clark, S.A. / Schraivogel, D. / Kallas, M. / Beckmann, R. / Tollervey, D. / Barbar, E. / Sinning, I. / Hurt, E. | ||||||
Citation | Journal: J.Cell Biol. / Year: 2014 Title: A network of assembly factors is involved in remodeling rRNA elements during preribosome maturation. Authors: Baler, J. / Paternoga, H. / Holdermann, I. / Thoms, M. / Granneman, S. / Barrio-Garcia, C. / Nyarko, A. / Stier, G. / Clark, S.A. / Schraivogel, D. / Kallas, M. / Beckmann, R. / Tollervey, D. ...Authors: Baler, J. / Paternoga, H. / Holdermann, I. / Thoms, M. / Granneman, S. / Barrio-Garcia, C. / Nyarko, A. / Stier, G. / Clark, S.A. / Schraivogel, D. / Kallas, M. / Beckmann, R. / Tollervey, D. / Barbar, E. / Sinning, I. / Hurt, E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2mvf.cif.gz | 576.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2mvf.ent.gz | 483.5 KB | Display | PDB format |
PDBx/mmJSON format | 2mvf.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mv/2mvf ftp://data.pdbj.org/pub/pdb/validation_reports/mv/2mvf | HTTPS FTP |
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-Related structure data
Related structure data | 4wjsC 4wjuC 4wjvC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10317.965 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719 / Gene: CTHT_0009080 / Production host: Escherichia coli (E. coli) / References: UniProt: G0S081 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 10 % D2O, 10 mM TRIS, 90 % H2O, 5 mM sodium chloride, 1 uM DSS, 2 mM TCEP, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | |||||||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 5 / pH: 7.4 / Pressure: ambient / Temperature: 280 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |