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Yorodumi- PDB-2mqh: Solution structure of the Chlamydomonas reinhardtii NAB1 cold sho... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2mqh | ||||||
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Title | Solution structure of the Chlamydomonas reinhardtii NAB1 cold shock domain, CSD1 | ||||||
Components | Nucleic acid binding protein | ||||||
Keywords | DNA BINDING PROTEIN / protein / cold shock domain / RNA-binding / Chlamydomonas reinhardtii / algae | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Chlamydomonas reinhardtii (plant) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Model details | lowest energy, model1 | ||||||
Authors | Sawyer, A. / Mobli, M. | ||||||
Citation | Journal: Biochem.J. / Year: 2015 Title: Solution structure of the RNA-binding cold-shock domain of the Chlamydomonas reinhardtii NAB1 protein and insights into RNA recognition. Authors: Sawyer, A.L. / Landsberg, M.J. / Ross, I.L. / Kruse, O. / Mobli, M. / Hankamer, B. #2: Journal: Plant Cell / Year: 2005 Title: NAB1 is an RNA binding protein involved in the light-regulated differential expression of the light-harvesting antenna of Chlamydomonas reinhardtii Authors: Mussgnug, J. / Wobbe, L. / Elles, I. / Claus, C. / Hamilton, M. / Fink, A. / Kahmann, U. / Kapazoglou, A. / Mullineaux, C. / Hippler, M. / Nickelsen, J. / Nixon, P. / Kruse, O. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2mqh.cif.gz | 480.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2mqh.ent.gz | 409.7 KB | Display | PDB format |
PDBx/mmJSON format | 2mqh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mq/2mqh ftp://data.pdbj.org/pub/pdb/validation_reports/mq/2mqh | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 8997.812 Da / Num. of mol.: 1 / Fragment: cold shock domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chlamydomonas reinhardtii (plant) Gene: CHLREDRAFT_126810, Nab1, NAB1 nucleic acid binding protein Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q8GV23 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: All non NOE 3D experiments acquired using NUS and processed using MaxEnt. |
-Sample preparation
Details | Contents: 267 uM [U-100% 13C; U-100% 15N] NAB1 cold shock domain, 20 mM sodium phosphate, 300 mM sodium chloride, 95% H2O/5% D2O Solvent system: 95% H2O/5% D2O | ||||||||||||||||
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Sample |
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Sample conditions |
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-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 900 MHz |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 / Details: Automated noe assignment | ||||||||||||||||||||||||||||||||
NMR constraints | NOE constraints total: 679 / NOE intraresidue total count: 192 / NOE long range total count: 194 / NOE medium range total count: 33 / NOE sequential total count: 260 | ||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |