+Open data
-Basic information
Entry | Database: PDB / ID: 2mob | ||||||
---|---|---|---|---|---|---|---|
Title | METHANE MONOOXYGENASE COMPONENT B | ||||||
Components | PROTEIN (METHANE MONOOXYGENASE REGULATORY PROTEIN B) | ||||||
Keywords | OXIDOREDUCTASE / MONOOXYGENASE / METHANE OXIDATION | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Methylosinus trichosporium (bacteria) | ||||||
Method | SOLUTION NMR | ||||||
Authors | Chang, S.L. / Wallar, B.J. / Lipscomb, J.D. / Mayo, K.H. | ||||||
Citation | Journal: Biochemistry / Year: 1999 Title: Solution structure of component B from methane monooxygenase derived through heteronuclear NMR and molecular modeling. Authors: Chang, S.L. / Wallar, B.J. / Lipscomb, J.D. / Mayo, K.H. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2mob.cif.gz | 428.1 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2mob.ent.gz | 350.1 KB | Display | PDB format |
PDBx/mmJSON format | 2mob.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mo/2mob ftp://data.pdbj.org/pub/pdb/validation_reports/mo/2mob | HTTPS FTP |
---|
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 14896.885 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Methylosinus trichosporium (bacteria) / Production host: Escherichia coli (E. coli) / References: UniProt: P27356 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
---|---|
NMR details | Text: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C, 15N-LABELED PROTEIN. |
-Sample preparation
Sample conditions | pH: 6.8 / Temperature: 313 K |
---|---|
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
|
---|
-Processing
NMR software | Name: X-PLOR / Version: 3.851 / Developer: BRUNGER / Classification: refinement |
---|---|
Refinement | Software ordinal: 1 Details: THESE STRUCTURES WERE CALCULATED BY X-PLOR 3.851 SIMULATED ANNEALING PROTOCOL ( NILGES ET AL., FEBS LETT. 229, 317-324) USING 1182 NOE, 98 PHI ANGLE, AND 60 HYDROGEN BONDING CONSTRAINTS. |
NMR ensemble | Conformers submitted total number: 15 |