+Open data
-Basic information
Entry | Database: PDB / ID: 2mhm | ||||||
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Title | Solution structure of cytochrome c Y67H | ||||||
Components | Cytochrome c iso-1 | ||||||
Keywords | ELECTRON TRANSPORT / Y67H / hydrogen-bond / H2O2 / guaiacol / peroxidation | ||||||
Function / homology | Function and homology information Release of apoptotic factors from the mitochondria / Pyroptosis / Detoxification of Reactive Oxygen Species / Respiratory electron transport / mitochondrial electron transport, cytochrome c to oxygen / mitochondrial electron transport, ubiquinol to cytochrome c / respirasome / mitochondrial intermembrane space / electron transfer activity / heme binding ...Release of apoptotic factors from the mitochondria / Pyroptosis / Detoxification of Reactive Oxygen Species / Respiratory electron transport / mitochondrial electron transport, cytochrome c to oxygen / mitochondrial electron transport, ubiquinol to cytochrome c / respirasome / mitochondrial intermembrane space / electron transfer activity / heme binding / mitochondrion / metal ion binding Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model7 | ||||||
Authors | Lan, W.X. / Wang, Z.H. / Yang, Z.Z. / Ying, T.L. / Wu, H.M. / Tan, X.S. / Cao, C.Y. / Huang, Z.X. | ||||||
Citation | Journal: Plos One / Year: 2014 Title: Structural Basis for Cytochrome c Y67H Mutant to Function as a Peroxidase Authors: Lan, W.X. / Wang, Z.H. / Yang, Z.Z. / Ying, T.L. / Zhang, X. / Tan, X.S. / Liu, M. / Cao, C.Y. / Huang, Z.X. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2mhm.cif.gz | 685 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2mhm.ent.gz | 592.8 KB | Display | PDB format |
PDBx/mmJSON format | 2mhm.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mh/2mhm ftp://data.pdbj.org/pub/pdb/validation_reports/mh/2mhm | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11988.666 Da / Num. of mol.: 1 / Mutation: Y67H Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Strain: S288c / Gene: CYC1, J1653, YJR048W / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: P00044 |
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#2: Chemical | ChemComp-HEC / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 50 / pH: 7.0 / Pressure: 1 atm / Temperature: 293 K |
-NMR measurement
NMR spectrometer | Type: Agilent NMR / Manufacturer: Agilent / Model: NMR / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||
NMR representative | Selection criteria: lowest energy | |||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 / Representative conformer: 7 |