+Open data
-Basic information
Entry | Database: PDB / ID: 2mdx | ||||||
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Title | Solution structure of the human wild type FAPP1-PH domain | ||||||
Components | Pleckstrin homology domain-containing family A member 3 | ||||||
Keywords | MEMBRANE PROTEIN / pleckstrin homology / phosphoinositide | ||||||
Function / homology | Function and homology information receptor recycling / endosome organization / retrograde transport, endosome to Golgi / phosphatidylinositol-4-phosphate binding / Synthesis of PIPs at the plasma membrane / trans-Golgi network / recycling endosome / early endosome / Golgi membrane / Golgi apparatus ...receptor recycling / endosome organization / retrograde transport, endosome to Golgi / phosphatidylinositol-4-phosphate binding / Synthesis of PIPs at the plasma membrane / trans-Golgi network / recycling endosome / early endosome / Golgi membrane / Golgi apparatus / identical protein binding / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / DGSA-distance geometry simulated annealing | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Lenoir, M. / Overduin, M. | ||||||
Citation | Journal: To be Published Title: Solution structure of the human wild type FAPP1-PH domain Authors: Lenoir, M. / Overduin, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2mdx.cif.gz | 335.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2mdx.ent.gz | 276.8 KB | Display | PDB format |
PDBx/mmJSON format | 2mdx.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/md/2mdx ftp://data.pdbj.org/pub/pdb/validation_reports/md/2mdx | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 12235.950 Da / Num. of mol.: 1 / Fragment: UNP residues 1-100 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: PLEKHA3, FAPP1 / Plasmid: pGex / Production host: Escherichia coli (E. coli) / References: UniProt: Q9HB20 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1 mM DTT, 20 mM TRIS, 100 mM sodium chloride, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||
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Sample |
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Sample conditions | Ionic strength: 240 / pH: 7 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: DGSA-distance geometry simulated annealing / Software ordinal: 1 Details: final stage of the calculations is refined in explicit water | ||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 10 |