+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 2m9f | ||||||
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タイトル | NMR solution structure of Pin1 WW domain mutant 5-1g | ||||||
要素 | Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 | ||||||
キーワード | ISOMERASE (異性化酵素) / N-glycosylation (N-結合型グリコシル化) / Enhanced Aromatic Sequon / WW domain (WWドメイン) / CH-pi interaction | ||||||
機能・相同性 | 機能・相同性情報 cis-trans isomerase activity / phosphothreonine residue binding / negative regulation of cell motility / ubiquitin ligase activator activity / regulation of protein localization to nucleus / GTPase activating protein binding / postsynaptic cytosol / mitogen-activated protein kinase kinase binding / regulation of mitotic nuclear division / negative regulation of SMAD protein signal transduction ...cis-trans isomerase activity / phosphothreonine residue binding / negative regulation of cell motility / ubiquitin ligase activator activity / regulation of protein localization to nucleus / GTPase activating protein binding / postsynaptic cytosol / mitogen-activated protein kinase kinase binding / regulation of mitotic nuclear division / negative regulation of SMAD protein signal transduction / PI5P Regulates TP53 Acetylation / negative regulation of amyloid-beta formation / cytoskeletal motor activity / RHO GTPases Activate NADPH Oxidases / phosphoserine residue binding / protein peptidyl-prolyl isomerization / positive regulation of protein dephosphorylation / ciliary basal body / regulation of cytokinesis / negative regulation of protein binding / プロリルイソメラーゼ / peptidyl-prolyl cis-trans isomerase activity / Negative regulators of DDX58/IFIH1 signaling / phosphoprotein binding / synapse organization / regulation of protein phosphorylation / negative regulation of transforming growth factor beta receptor signaling pathway / regulation of protein stability / tau protein binding / neuron differentiation / negative regulation of protein catabolic process / negative regulation of ERK1 and ERK2 cascade / ISG15 antiviral mechanism / beta-catenin binding / positive regulation of GTPase activity / positive regulation of canonical Wnt signaling pathway / positive regulation of protein binding / midbody / 遺伝子発現の調節 / Regulation of TP53 Activity through Phosphorylation / protein stabilization / response to hypoxia / nuclear speck / positive regulation of protein phosphorylation / 細胞周期 / glutamatergic synapse / positive regulation of transcription by RNA polymerase II / 核質 / 細胞核 / 細胞質基質 / 細胞質 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | 溶液NMR / molecular dynamics | ||||||
データ登録者 | Enck, S. / Chen, W. / Price, J.L. / Powers, E.T. / Wong, C. / Dyson, H.J. / Kelly, J.W. | ||||||
引用 | ジャーナル: J.Am.Chem.Soc. / 年: 2013 タイトル: Structural and energetic basis of carbohydrate-aromatic packing interactions in proteins. 著者: Chen, W. / Enck, S. / Price, J.L. / Powers, D.L. / Powers, E.T. / Wong, C.H. / Dyson, H.J. / Kelly, J.W. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 2m9f.cif.gz | 219.3 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb2m9f.ent.gz | 183 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 2m9f.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/m9/2m9f ftp://data.pdbj.org/pub/pdb/validation_reports/m9/2m9f | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質・ペプチド | 分子量: 3849.313 Da / 分子数: 1 断片: modified WW domain (UNP residues 6-39, see remark 999) 由来タイプ: 合成 / 由来: (合成) Homo sapiens (ヒト) / 参照: UniProt: Q13526 |
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#2: 糖 | ChemComp-NAG / |
配列の詳細 | SYNTHESIZE |
-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||
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NMR実験 |
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-試料調製
詳細 | 内容: 500 uM Pin WW Domain Peptide, 50 mM sodium phosphate, 90% H2O/10% D2O 溶媒系: 90% H2O/10% D2O | |||||||||
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試料 |
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試料状態 | イオン強度: 0.08 / pH: 6.5 / 圧: ambient / 温度: 285 K |
-NMR測定
NMRスペクトロメーター |
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-解析
NMR software |
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精密化 | 手法: molecular dynamics / ソフトェア番号: 1 | |||||||||
NMR constraints | NOE constraints total: 328 / NOE intraresidue total count: 90 / NOE long range total count: 121 / NOE medium range total count: 47 / NOE sequential total count: 70 | |||||||||
代表構造 | 選択基準: lowest energy | |||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 200 / 登録したコンフォーマーの数: 20 |