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Yorodumi- PDB-2m7v: Green Light-Absorbing State of TePixJ, an Active Cyanobacteriochr... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2m7v | ||||||
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Title | Green Light-Absorbing State of TePixJ, an Active Cyanobacteriochrome Domain | ||||||
Components | Methyl-accepting chemotaxis proteinMethyl-accepting chemotaxis proteins | ||||||
Keywords | SIGNALING PROTEIN / phytochrome / PixJ / blue/green light-absorbing / cyanobacteria / PVB / phycoviolobilin | ||||||
Function / homology | Function and homology information chemotaxis / lysozyme activity / signal transduction / metal ion binding / plasma membrane Similarity search - Function | ||||||
Biological species | Thermosynechococcus elongatus (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing, torsion angle dynamics | ||||||
Model details | lowest energy, model1 | ||||||
Authors | Cornilescu, G. / Cornilescu, C.C. / Burgie, S.E. / Walker, J.M. / Markley, J.L. / Ulijasz, A.T. / Vierstra, R.D. | ||||||
Citation | Journal: To be Published Title: Structural and Flexibility Variations Between the Ground and Photoactivated States of a Blue/Green Light-Absorbing Cyanobacteriochrome Authors: Cornilescu, C.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2m7v.cif.gz | 535.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2m7v.ent.gz | 451.6 KB | Display | PDB format |
PDBx/mmJSON format | 2m7v.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m7/2m7v ftp://data.pdbj.org/pub/pdb/validation_reports/m7/2m7v | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 18769.307 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermosynechococcus elongatus (bacteria) Strain: BP-1 / Gene: tll0569 / Production host: Escherichia coli (E. coli) / References: UniProt: Q8DLC7 |
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#2: Chemical | ChemComp-PVN / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR / Details: Pg state | ||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 0.7-1.3 mM [U-13C; U-15N] TePixJ, 0.7-1.3 mM PHYCOVIOLOBILIN, 93% H2O/7% D2O Solvent system: 93% H2O/7% D2O | |||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 10 / pH: 8 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing, torsion angle dynamics / Software ordinal: 1 | |||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||
NMR ensemble | Conformer selection criteria: back calculated data agree with experimental NOESY spectrum Conformers calculated total number: 100 / Conformers submitted total number: 10 |