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- PDB-2m56: The structure of the complex of cytochrome P450cam and its electr... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2m56 | ||||||
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Title | The structure of the complex of cytochrome P450cam and its electron donor putidaredoxin determined by paramagnetic NMR spectroscopy | ||||||
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![]() | OXIDOREDUCTASE/METAL BINDING PROTEIN / ![]() ![]() ![]() ![]() ![]() ![]() | ||||||
Function / homology | ![]() P450-containing electron transport chain / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
Model details | closest to the average, model1 | ||||||
![]() | Hiruma, Y. / Hass, M.A.S. / Ubbink, M. | ||||||
![]() | ![]() Title: The structure of the cytochrome p450cam-putidaredoxin complex determined by paramagnetic NMR spectroscopy and crystallography. Authors: Hiruma, Y. / Hass, M.A. / Kikui, Y. / Liu, W.M. / Olmez, B. / Skinner, S.P. / Blok, A. / Kloosterman, A. / Koteishi, H. / Lohr, F. / Schwalbe, H. / Nojiri, M. / Ubbink, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 1.5 MB | Display | ![]() |
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PDB format | ![]() | 1.3 MB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | ![]() Mass: 45527.832 Da / Num. of mol.: 1 / Fragment: UNP RESIDUES 12-415 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Description: Xho1/Nco1 restriction sites, Kanamycin resistance Gene: camC, cyp101 / Production host: ![]() ![]() ![]() ![]() |
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#2: Protein | Mass: 11412.846 Da / Num. of mol.: 1 / Mutation: C74S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Description: Xho1/Nco1 restriction sites, Kanamycin resistance Gene: camB / Production host: ![]() ![]() ![]() |
#3: Chemical | ChemComp-HEM / ![]() |
#4: Chemical | ChemComp-CAM / ![]() |
#5: Chemical | ChemComp-FES / ![]() |
Sequence details | THE THE EXPERIMENT |
-Experimental details
-Experiment
Experiment | Method: ![]() | ||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: 100 uM [U-95% 15N] entity_1-1, 93% H2O/7% D2O / Solvent system: 93% H2O/7% D2O |
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Sample | Conc.: 100 uM / Component: entity_1-1 / Isotopic labeling: [U-95% 15N] |
Sample conditions | Ionic strength: 100 / pH: 7.4 / Pressure: ambient / Temperature: 290 K |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: ![]() | |||||||||
NMR representative | Selection criteria: closest to the average | |||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 10 / Representative conformer: 1 |