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Yorodumi- PDB-2lq5: NMR structure of the RNA binding motif 39 (RBM39) from Mus musculus -
+Open data
-Basic information
Entry | Database: PDB / ID: 2lq5 | ||||||
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Title | NMR structure of the RNA binding motif 39 (RBM39) from Mus musculus | ||||||
Components | RNA-binding protein 39 | ||||||
Keywords | RNA BINDING PROTEIN / RNA binding domain / Structural Genomics / PSI-Biology / Joint Center for Structural Genomics / JCSG / Partnership for T-Cell Biology / TCELL | ||||||
Function / homology | Function and homology information Nuclear Envelope Breakdown / Depolymerization of the Nuclear Lamina / RND3 GTPase cycle / Initiation of Nuclear Envelope (NE) Reformation / RAC3 GTPase cycle / RAC2 GTPase cycle / RS domain binding / RHOD GTPase cycle / mRNA Splicing - Major Pathway / RAC1 GTPase cycle ...Nuclear Envelope Breakdown / Depolymerization of the Nuclear Lamina / RND3 GTPase cycle / Initiation of Nuclear Envelope (NE) Reformation / RAC3 GTPase cycle / RAC2 GTPase cycle / RS domain binding / RHOD GTPase cycle / mRNA Splicing - Major Pathway / RAC1 GTPase cycle / RND2 GTPase cycle / RND1 GTPase cycle / RHOG GTPase cycle / U1 snRNP binding / regulation of mRNA splicing, via spliceosome / centriolar satellite / RNA splicing / mRNA processing / microtubule cytoskeleton / nuclear speck / protein-containing complex / RNA binding / nucleoplasm Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | SOLUTION NMR / molecular dynamics | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Serrano, P. / Dutta, S.K. / Geralt, M. / Wuthrich, K. / Joint Center for Structural Genomics (JCSG) / Partnership for T-Cell Biology (TCELL) | ||||||
Citation | Journal: To be Published Title: NMR structure of the RNA binding motif 39 (RBM39) from Mus musculus Authors: Serrano, P. / Dutta, S.K. / Geralt, M. / Wuthrich, K. / Joint Center for Structural Genomics (JCSG) / Partnership for T-Cell Biology (TCELL) | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2lq5.cif.gz | 718.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2lq5.ent.gz | 608.4 KB | Display | PDB format |
PDBx/mmJSON format | 2lq5.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lq/2lq5 ftp://data.pdbj.org/pub/pdb/validation_reports/lq/2lq5 | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 12516.202 Da / Num. of mol.: 1 / Fragment: RRM 3 domain residues 418-530 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Rbm39, Caper, Rnpc2 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 DE3 / References: UniProt: Q8VH51 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.2 mM [U-95% 13C; U-95% 15N] protein, 4.5 mM sodium azide, 50 mM sodium chloride, 25 mM sodium phosphate, 95% H2O/5% D2O Solvent system: 95% H2O/5% D2O | ||||||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 0.083 / pH: 6.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: molecular dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 80 / Conformers submitted total number: 20 |