+Open data
-Basic information
Entry | Database: PDB / ID: 2loe | ||||||
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Title | Structure of the Plasmodium 6-cysteine s48/45 Domain | ||||||
Components | 6-cysteine protein, putative | ||||||
Keywords | MEMBRANE PROTEIN / Malaria | ||||||
Function / homology | Function and homology information symbiont-containing vacuolar space / : / symbiont entry into host / side of membrane / apical part of cell / cell surface / plasma membrane Similarity search - Function | ||||||
Biological species | Plasmodium falciparum 3D7 (eukaryote) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | closest to the average, model 1 | ||||||
Authors | Cai, M. / Arredondo, S.A. / Clore, M.G. / Miller, L.H. / Takayama, Y. / Macdonald, N.J. / Enderson, E.D. / Aravind, L. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2012 Title: Structure of the Plasmodium 6-cysteine s48/45 domain. Authors: Arredondo, S.A. / Cai, M. / Takayama, Y. / Macdonald, N.J. / Anderson, D.E. / Aravind, L. / Clore, G.M. / Miller, L.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2loe.cif.gz | 765.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2loe.ent.gz | 647.8 KB | Display | PDB format |
PDBx/mmJSON format | 2loe.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lo/2loe ftp://data.pdbj.org/pub/pdb/validation_reports/lo/2loe | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 14410.165 Da / Num. of mol.: 1 / Fragment: UNP residues 174-300 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Plasmodium falciparum 3D7 (eukaryote) / Gene: Pf12, PFF0615c / Production host: Escherichia coli (E. coli) / References: UniProt: C6KSX0, UniProt: P19259*PLUS |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR Details: Solution structure of the Plasmodium 6-cysteine s48/45 Domain | ||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 0.4 mM [U-99% 13C; U-99% 15N] Pf1 phage, 95% H2O/5% D2O Solvent system: 95% H2O/5% D2O |
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Sample | Conc.: 0.4 mM / Component: Pf1 phage-1 / Isotopic labeling: [U-99% 13C; U-99% 15N] |
Sample conditions | Ionic strength: 0 / pH: 5.0 / Pressure: ambient / Temperature: 308 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||||||||
NMR constraints | NOE constraints total: 1543 | |||||||||||||||
NMR representative | Selection criteria: closest to the average | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |