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Yorodumi- PDB-2lh9: NMR structure of the first lotus domain of tudor domain-containin... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2lh9 | ||||||
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Title | NMR structure of the first lotus domain of tudor domain-containing protein 7 | ||||||
Components | Tudor domain-containing protein 7 | ||||||
Keywords | RNA BINDING PROTEIN / RNA binding domain / Ribonucleoprotein / RNA granule component | ||||||
Function / homology | Function and homology information P granule organization / ribonucleoprotein granule / piRNA processing / lens morphogenesis in camera-type eye / chromatoid body / lens fiber cell differentiation / P granule / post-transcriptional regulation of gene expression / germ cell development / spermatogenesis ...P granule organization / ribonucleoprotein granule / piRNA processing / lens morphogenesis in camera-type eye / chromatoid body / lens fiber cell differentiation / P granule / post-transcriptional regulation of gene expression / germ cell development / spermatogenesis / ribonucleoprotein complex / mRNA binding / cytoplasm Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Cui, G. / Botuyan, M. / Mer, G. | ||||||
Citation | Journal: To be Published Title: NMR structure of Tudor domain-containing protein 7 Authors: Cui, G. / Botuyan, M. / Mer, G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2lh9.cif.gz | 484.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2lh9.ent.gz | 406 KB | Display | PDB format |
PDBx/mmJSON format | 2lh9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lh/2lh9 ftp://data.pdbj.org/pub/pdb/validation_reports/lh/2lh9 | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 8681.102 Da / Num. of mol.: 1 / Fragment: sequence database residues 1-76 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Tdrd7, Pctaire2bp / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) Rosetta / References: UniProt: Q8K1H1 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 0 / pH: 4.3 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 700 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |