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Yorodumi- PDB-2lae: NMR solution structure of the C-terminal domain of the E. coli li... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2lae | ||||||
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Title | NMR solution structure of the C-terminal domain of the E. coli lipoprotein BamC | ||||||
Components | Lipoprotein 34 | ||||||
Keywords | MEMBRANE PROTEIN / BETA-BARREL ASSEMBLY MACHINERY / BAM / BAMC | ||||||
Function / homology | Function and homology information Bam protein complex / Gram-negative-bacterium-type cell outer membrane assembly / protein insertion into membrane / cell surface / membrane / identical protein binding Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | SOLUTION NMR / molecular dynamics | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Pardi, A. / Warner, L. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2011 Title: Structure of the BamC Two-Domain Protein Obtained by Rosetta with a Limited NMR Data Set. Authors: Warner, L.R. / Varga, K. / Lange, O.F. / Baker, S.L. / Baker, D. / Sousa, M.C. / Pardi, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2lae.cif.gz | 322.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2lae.ent.gz | 259.7 KB | Display | PDB format |
PDBx/mmJSON format | 2lae.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/la/2lae ftp://data.pdbj.org/pub/pdb/validation_reports/la/2lae | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 26998.979 Da / Num. of mol.: 1 / Fragment: sequence database residues 229-344 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K12 / Gene: nlpB, dapX, b2477, JW2462 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A903 |
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Compound details | THE ENTIRE PROTEIN SEQUENCE IN PROVIDED IN THE SEQRES SECTION OF THE PDB FILE. HOWEVER, THE ...THE ENTIRE PROTEIN SEQUENCE IN PROVIDED IN THE SEQRES SECTION OF THE PDB FILE. HOWEVER, THE ENSEMBLES WERE CALCULATED |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 0.2 / pH: 6.6 / Pressure: ambient / Temperature: 303 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: molecular dynamics / Software ordinal: 1 | |||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 9 |