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Yorodumi- PDB-2l97: Solution structure of HtrA PDZ domain from Streptococcus pneumoniae -
+Open data
-Basic information
Entry | Database: PDB / ID: 2l97 | ||||||
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Title | Solution structure of HtrA PDZ domain from Streptococcus pneumoniae | ||||||
Components | Putative serine protease | ||||||
Keywords | PROTEIN BINDING / HtrA-PDZ | ||||||
Function / homology | Function and homology information metallopeptidase activity / serine-type endopeptidase activity / plasma membrane Similarity search - Function | ||||||
Biological species | Streptococcus pneumoniae (bacteria) | ||||||
Method | SOLUTION NMR / distance geometry | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Fan, K. / Zhang, J. / Zhang, X. / Tu, X. | ||||||
Citation | Journal: J.Struct.Biol. / Year: 2011 Title: Solution structure of HtrA PDZ domain from Streptococcus pneumoniae and its interaction with YYF-COOH containing peptides. Authors: Fan, K. / Zhang, J. / Zhang, X. / Tu, X. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2l97.cif.gz | 889 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2l97.ent.gz | 765.6 KB | Display | PDB format |
PDBx/mmJSON format | 2l97.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/l9/2l97 ftp://data.pdbj.org/pub/pdb/validation_reports/l9/2l97 | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 14890.758 Da / Num. of mol.: 1 / Fragment: PDZ domain, UNP residues 266-390 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptococcus pneumoniae (bacteria) / Production host: Escherichia coli (E. coli) / References: UniProt: O06670, UniProt: A0A0H2US63*PLUS |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 0.6mM [U-99% 13C; U-99% 15N] HtrA-PDZ; 100mM sodium chloride; 25mM sodium phosphate; 2mM DTT; 1.5mM EDTA; 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 0.1 / pH: 6.8 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker DMX / Manufacturer: Bruker / Model: DMX / Field strength: 500 MHz |
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-Processing
NMR software |
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Refinement | Method: distance geometry / Software ordinal: 1 | |||||||||
NMR representative | Selection criteria: lowest energy | |||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 / Representative conformer: 1 |