+Open data
-Basic information
Entry | Database: PDB / ID: 2l4g | ||||||
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Title | Influenza Haemagglutinin fusion peptide mutant G13A | ||||||
Components | HaemagglutininHemagglutinin | ||||||
Keywords | VIRAL PROTEIN / fusion peptide | ||||||
Function / homology | Function and homology information viral budding from plasma membrane / clathrin-dependent endocytosis of virus by host cell / membrane => GO:0016020 / host cell surface receptor binding / apical plasma membrane / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane Similarity search - Function | ||||||
Biological species | Influenza A virus | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | closest to the average, model 1 | ||||||
Authors | Lai, A.L. / Tamm, L.K. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2010 Title: Shallow boomerang-shaped influenza hemagglutinin G13A mutant structure promotes leaky membrane fusion. Authors: Lai, A.L. / Tamm, L.K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2l4g.cif.gz | 100.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2l4g.ent.gz | 68.8 KB | Display | PDB format |
PDBx/mmJSON format | 2l4g.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/l4/2l4g ftp://data.pdbj.org/pub/pdb/validation_reports/l4/2l4g | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 2802.276 Da / Num. of mol.: 1 / Fragment: UNP residues 346-365 / Mutation: G13A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Influenza A virus / Strain: X31 / Plasmid: pET31b+ / Production host: Escherichia coli (E. coli) / References: UniProt: Q9DHG0 |
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Sequence details | THIS PORTION OF THE SEQUENCE (-GCGKKKK) REPRESENTS A SEGMENT INTRODUCED TO INCREASE THE SOLUBILITY ...THIS PORTION OF THE SEQUENCE (-GCGKKKK) REPRESENTS |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | pH: 5 / Pressure: ambient / Temperature: 303 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 40 / Conformers submitted total number: 20 |