+Open data
-Basic information
Entry | Database: PDB / ID: 2kwh | ||||||
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Title | Ral binding domain of RLIP76 (RalBP1) | ||||||
Components | RalA-binding protein 1 | ||||||
Keywords | TRANSPORT PROTEIN / GTPase activation | ||||||
Function / homology | Function and homology information doxorubicin transport / ABC-type glutathione-S-conjugate transporter / ABC-type glutathione S-conjugate transporter activity / xenobiotic detoxification by transmembrane export across the plasma membrane / ABC-type xenobiotic transporter / ABC-type xenobiotic transporter activity / regulation of Cdc42 protein signal transduction / regulation of small GTPase mediated signal transduction / positive regulation of mitochondrial fission / small GTPase-mediated signal transduction ...doxorubicin transport / ABC-type glutathione-S-conjugate transporter / ABC-type glutathione S-conjugate transporter activity / xenobiotic detoxification by transmembrane export across the plasma membrane / ABC-type xenobiotic transporter / ABC-type xenobiotic transporter activity / regulation of Cdc42 protein signal transduction / regulation of small GTPase mediated signal transduction / positive regulation of mitochondrial fission / small GTPase-mediated signal transduction / regulation of GTPase activity / xenobiotic transmembrane transporter activity / CDC42 GTPase cycle / transmembrane transporter activity / ATPase-coupled transmembrane transporter activity / RAC1 GTPase cycle / GTPase activator activity / transmembrane transport / small GTPase binding / positive regulation of GTPase activity / spindle pole / endocytosis / chemotaxis / nuclear body / positive regulation of protein phosphorylation / mitochondrion / nucleoplasm / ATP binding / membrane / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | closest to the average, model 1 | ||||||
Authors | Fenwick, R.B. / Campbell, L.J. / Rajasekar, K. / Prasannan, S. / Nietlispach, D. / Camonis, J. / Owen, D. / Mott, H.R. | ||||||
Citation | Journal: Structure / Year: 2010 Title: The RalB-RLIP76 complex reveals a novel mode of ral-effector interaction Authors: Fenwick, R.B. / Campbell, L.J. / Rajasekar, K. / Prasannan, S. / Nietlispach, D. / Camonis, J. / Owen, D. / Mott, H.R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2kwh.cif.gz | 904.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2kwh.ent.gz | 763.7 KB | Display | PDB format |
PDBx/mmJSON format | 2kwh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kw/2kwh ftp://data.pdbj.org/pub/pdb/validation_reports/kw/2kwh | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 6600.567 Da / Num. of mol.: 1 / Fragment: UNP residues 393-446 / Mutation: C411S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: RALBP1, RLIP1, RLIP76 / Production host: Escherichia coli (E. coli) / References: UniProt: Q15311 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 0.15 / pH: 6.3 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 500 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 50 |