+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 2kkw | ||||||
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タイトル | SLAS-micelle bound alpha-synuclein | ||||||
要素 | Alpha-synucleinΑ-シヌクレイン | ||||||
キーワード | LIPID BINDING PROTEIN / Protein-micelle interaction | ||||||
機能・相同性 | 機能・相同性情報 negative regulation of mitochondrial electron transport, NADH to ubiquinone / neutral lipid metabolic process / regulation of phospholipase activity / negative regulation of monooxygenase activity / regulation of acyl-CoA biosynthetic process / negative regulation of dopamine uptake involved in synaptic transmission / negative regulation of norepinephrine uptake / positive regulation of glutathione peroxidase activity / positive regulation of SNARE complex assembly / positive regulation of hydrogen peroxide catabolic process ...negative regulation of mitochondrial electron transport, NADH to ubiquinone / neutral lipid metabolic process / regulation of phospholipase activity / negative regulation of monooxygenase activity / regulation of acyl-CoA biosynthetic process / negative regulation of dopamine uptake involved in synaptic transmission / negative regulation of norepinephrine uptake / positive regulation of glutathione peroxidase activity / positive regulation of SNARE complex assembly / positive regulation of hydrogen peroxide catabolic process / supramolecular fiber / negative regulation of transporter activity / mitochondrial membrane organization / negative regulation of chaperone-mediated autophagy / regulation of reactive oxygen species biosynthetic process / positive regulation of protein localization to cell periphery / regulation of synaptic vesicle recycling / negative regulation of platelet-derived growth factor receptor signaling pathway / negative regulation of exocytosis / regulation of glutamate secretion / response to iron(II) ion / regulation of norepinephrine uptake / dopamine biosynthetic process / SNARE complex assembly / positive regulation of neurotransmitter secretion / regulation of locomotion / positive regulation of inositol phosphate biosynthetic process / synaptic vesicle priming / regulation of macrophage activation / dopamine uptake involved in synaptic transmission / negative regulation of microtubule polymerization / synaptic vesicle transport / dynein complex binding / positive regulation of receptor recycling / regulation of dopamine secretion / protein kinase inhibitor activity / negative regulation of thrombin-activated receptor signaling pathway / response to type II interferon / cuprous ion binding / positive regulation of exocytosis / synaptic vesicle exocytosis / mitochondrial ATP synthesis coupled electron transport / positive regulation of endocytosis / kinesin binding / cysteine-type endopeptidase inhibitor activity involved in apoptotic process / response to magnesium ion / synaptic vesicle endocytosis / regulation of presynapse assembly / alpha-tubulin binding / negative regulation of serotonin uptake / localization / phospholipid metabolic process / axon terminus / supramolecular fiber organization / 封入体 / cellular response to copper ion / Hsp70 protein binding / cellular response to epinephrine stimulus / excitatory postsynaptic potential / response to interleukin-1 / adult locomotory behavior / SNARE binding / positive regulation of release of sequestered calcium ion into cytosol / fatty acid metabolic process / long-term synaptic potentiation / regulation of transmembrane transporter activity / phosphoprotein binding / protein tetramerization / synapse organization / regulation of long-term neuronal synaptic plasticity / microglial cell activation / negative regulation of protein kinase activity / ferrous iron binding / protein destabilization / PKR-mediated signaling / negative regulation of cysteine-type endopeptidase activity involved in apoptotic process / tau protein binding / positive regulation of protein serine/threonine kinase activity / receptor internalization / phospholipid binding / synaptic vesicle membrane / positive regulation of inflammatory response / activation of cysteine-type endopeptidase activity involved in apoptotic process / マイクロフィラメント / positive regulation of peptidyl-serine phosphorylation / actin binding / cellular response to oxidative stress / 細胞皮質 / histone binding / 成長円錐 / postsynapse / chemical synaptic transmission / neuron apoptotic process / negative regulation of neuron apoptotic process / response to lipopolysaccharide / amyloid fibril formation / リソソーム / molecular adaptor activity / oxidoreductase activity / transcription cis-regulatory region binding 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | 溶液NMR / EPR / simulated annealing | ||||||
Model details | most populated ensemble subset representative, model 1 | ||||||
データ登録者 | Rao, J. / Jao, C.C. / Hegde, B. / Langen, R. / Ulmer, T.S. | ||||||
引用 | ジャーナル: J.Am.Chem.Soc. / 年: 2010 タイトル: A combinatorial NMR and EPR approach for evaluating the structural ensemble of partially folded proteins. 著者: Rao, J.N. / Jao, C.C. / Hegde, B.G. / Langen, R. / Ulmer, T.S. | ||||||
履歴 |
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Remark 650 | HELIX DETERMINATION METHOD: AUTHOR DETERMINED |
-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 2kkw.cif.gz | 1.3 MB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb2kkw.ent.gz | 1.1 MB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 2kkw.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/kk/2kkw ftp://data.pdbj.org/pub/pdb/validation_reports/kk/2kkw | HTTPS FTP |
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-関連構造データ
類似構造データ | |
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その他のデータベース |
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 14476.108 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: SNCA, NACP, PARK1 / プラスミド: pET-42 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P37840 |
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-実験情報
-実験
実験 |
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NMR実験 |
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NMR実験の詳細 | Text: EPR EXPERIMENT WAS DONE USING ELEXSYS E 580 SPECTROMETER AT 0.45 T, 78K, AMBIENT PRESSURE, PH 7.4, IONIC STRENGHT 0.1. THE EPR SAMPLE CONTAINED 50 UM ALPHA-SYNUCLEIN, 5 MM SODIUM LAUROYL SARCOSINATE, 30% SUCROSE |
-試料調製
詳細 | 内容: 0.75 MM [U-100% 13C, U-100% 15N, U-80% 2H] ALPHA-SYNUCLEIN, 75 MM SODIUM LAUROYL SARCOSINATE, 95% H2O/5% D2O | ||||||||||||||||||||||||
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試料 |
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試料状態 | イオン強度: 0.05 / pH: 7.4 / 圧: AMBIENT / 温度: 298.2 K |
-データ収集
NMRスペクトロメーター | タイプ: Bruker AVANCE / 製造業者: Bruker / モデル: AVANCE / 磁場強度: 700 MHz |
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-解析
NMR software |
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精密化 | 手法: simulated annealing / ソフトェア番号: 1 | |||||||||
NMRアンサンブル | コンフォーマー選択の基準: ENSEMBLE SUBPOPULATION AVERAGE REPRESENTATIVES 計算したコンフォーマーの数: 85000 / 登録したコンフォーマーの数: 34 |