+Open data
-Basic information
Entry | Database: PDB / ID: 2kbp | ||||||||||||||||||
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Title | Solution structure of a G-quadruplex of human telomeric RNA | ||||||||||||||||||
Components | 5'-R(*Keywords | RNA / G-quadruplex / parallel form / telomeric | Function / homology | RNA / RNA (> 10) | Function and homology information Method | SOLUTION NMR / Distance geometry, simulated annealing, molecular dynamics constrained with NMR data (NOE, dihedral, hydrogen-bond constraints) | Authors | Phan, A.T. / Martadinata, H. | Citation | Journal: J.Am.Chem.Soc. / Year: 2009 | Title: Structure of propeller-type parallel-stranded RNA G-quadruplexes, formed by human telomeric RNA sequences in K+ solution. Authors: Martadinata, H. / Phan, A.T. History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2kbp.cif.gz | 161.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2kbp.ent.gz | 135.9 KB | Display | PDB format |
PDBx/mmJSON format | 2kbp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kb/2kbp ftp://data.pdbj.org/pub/pdb/validation_reports/kb/2kbp | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: RNA chain | Mass: 3909.352 Da / Num. of mol.: 2 / Source method: obtained synthetically |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.25mM telomeric RNA-1, 70 mM potassium chloride-2, 20 mM potassium phosphate-3, 10 % D2O-4, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | |||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 90 mM K+ / pH: 7.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: Distance geometry, simulated annealing, molecular dynamics constrained with NMR data (NOE, dihedral, hydrogen-bond constraints) Software ordinal: 1 | |||||||||
NMR representative | Selection criteria: lowest energy | |||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 10 / Representative conformer: 1 |