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Yorodumi- PDB-2k2u: NMR Structure of the complex between Tfb1 subunit of TFIIH and th... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2k2u | ||||||
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Title | NMR Structure of the complex between Tfb1 subunit of TFIIH and the activation domain of VP16 | ||||||
Components |
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Keywords | TRANSCRIPTION / VP16 / TFIIH / Tfb1 / Activation / PH Domain / Protein Structure Complex / DNA damage / DNA repair / Nucleus / Transcription regulation / DNA-binding | ||||||
Function / homology | Function and homology information DNA-templated viral transcription / viral tegument / phosphatidylinositol-5-phosphate binding / nucleotide-excision repair factor 3 complex / phosphatidylinositol-3-phosphate binding / transcription factor TFIIH core complex / transcription factor TFIIH holo complex / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / mRNA Capping ...DNA-templated viral transcription / viral tegument / phosphatidylinositol-5-phosphate binding / nucleotide-excision repair factor 3 complex / phosphatidylinositol-3-phosphate binding / transcription factor TFIIH core complex / transcription factor TFIIH holo complex / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / mRNA Capping / TP53 Regulates Transcription of DNA Repair Genes / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA Polymerase II Pre-transcription Events / Formation of TC-NER Pre-Incision Complex / RNA Polymerase I Promoter Escape / Gap-filling DNA repair synthesis and ligation in TC-NER / Dual incision in TC-NER / transcription by RNA polymerase I / transcription initiation at RNA polymerase II promoter / nucleotide-excision repair / transcription by RNA polymerase II / DNA repair / host cell nucleus / regulation of DNA-templated transcription / DNA binding / nucleus / cytosol Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) Human herpesvirus 1 (Herpes simplex virus type 1) | ||||||
Method | SOLUTION NMR / simulated annealing, torsion angle dynamics, simulated annealing | ||||||
Authors | Langlois, C. / Mas, C. / Di Lello, P. / Miller Jenkins, P.M. / Legault, J. / Omichinski, J.G. | ||||||
Citation | Journal: J.Am.Chem.Soc. / Year: 2008 Title: NMR Structure of the Complex between the Tfb1 Subunit of TFIIH and the Activation Domain of VP16: Structural Similarities between VP16 and p53. Authors: Langlois, C. / Mas, C. / Di Lello, P. / Jenkins, L.M. / Legault, P. / Omichinski, J.G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2k2u.cif.gz | 902.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2k2u.ent.gz | 755.8 KB | Display | PDB format |
PDBx/mmJSON format | 2k2u.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/k2/2k2u ftp://data.pdbj.org/pub/pdb/validation_reports/k2/2k2u | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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NMR ensembles |
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-Components
#1: Protein | Mass: 12903.701 Da / Num. of mol.: 1 / Fragment: PH domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Species: Cerevisiae / Gene: TFB1, YDR311W, D9740.3 / Plasmid: plasmid / Species (production host): coli / Production host: Escherichia coli (E. coli) / Strain (production host): TOPP2 / References: UniProt: P32776 |
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#2: Protein/peptide | Mass: 3817.064 Da / Num. of mol.: 1 / Fragment: Transcription activation domain 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Human herpesvirus 1 (Herpes simplex virus type 1) Strain: strain F / Gene: UL48 / Plasmid: plasmid / Species (production host): coli / Production host: Escherichia coli (E. coli) / Strain (production host): TOPP2 / References: UniProt: P04486 |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 20mM Sodium Phosphate / pH: 6.5 / Pressure: Ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software | Name: CNS / Developer: Brunger A. T. et.al. / Classification: refinement |
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Refinement | Method: simulated annealing, torsion angle dynamics, simulated annealing Software ordinal: 1 Details: The three-dimensional structures of the complex Tfb1/VP16 were determined using a set of 1568 NOE-derived distance restraints, 158 backbone dihedral angle (Phi and Psi)restraints and 36 ...Details: The three-dimensional structures of the complex Tfb1/VP16 were determined using a set of 1568 NOE-derived distance restraints, 158 backbone dihedral angle (Phi and Psi)restraints and 36 distance restraints from hydrogen bounds., The three-dimensional structures of the complex Tfb1/VP16 were determined using a set of 1568 NOE-derived distance restraints, 158 backbone dihedral angle (Phi and Psi)restraints and 36 distance restraints fron hydrogen bounds. |
NMR constraints | NOE constraints total: 1568 / NOE intraresidue total count: 578 / NOE long range total count: 309 / NOE medium range total count: 194 / NOE sequential total count: 446 / Protein phi angle constraints total count: 79 / Protein psi angle constraints total count: 79 |
NMR representative | Selection criteria: lowest energy |
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |
NMR ensemble rms | Bond angle rms dev: 0.395 ° / Covalent bond rms dev: 0.0025 Å / Dihedral angles rms dev: 29.899 ° / Improper torsion angle rms dev: 0.281 ° |