+Open data
-Basic information
Entry | Database: PDB / ID: 2k0s | ||||||
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Title | Solution NMR structure of protein BC066483 | ||||||
Components | ADP-ribosylation factor-like protein 2-binding protein | ||||||
Keywords | STRUCTURAL PROTEIN / protein / Cytoplasm / Mitochondrion / Structural Genomics / PSI-2 / Protein Structure Initiative / Center for Eukaryotic Structural Genomics / CESG | ||||||
Function / homology | Function and homology information Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane / maintenance of protein location in nucleus / microtubule organizing center / positive regulation of tyrosine phosphorylation of STAT protein / mitochondrial intermembrane space / cilium / spindle / nucleus Similarity search - Function | ||||||
Biological species | Danio rerio (zebrafish) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Cornilescu, C.C. / Loushin-Newman, C.L. / Vinarov, D.A. / Markley, J.L. / Center for Eukaryotic Structural Genomics (CESG) | ||||||
Citation | Journal: To be Published Title: Solution NMR structure of ADP-ribosylation factor-like protein 2-binding protein. Authors: Cornilescu, C.C. / Loushin-Newman, C.L. / Vinarov, D.A. / Markley, J.L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2k0s.cif.gz | 213.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2k0s.ent.gz | 176.4 KB | Display | PDB format |
PDBx/mmJSON format | 2k0s.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/k0/2k0s ftp://data.pdbj.org/pub/pdb/validation_reports/k0/2k0s | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 15549.195 Da / Num. of mol.: 1 / Fragment: Residues 19-139 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Danio rerio (zebrafish) / Description: Wheat germ cell-free, in vitro expression / Gene: arl2bp / Plasmid: pEU-HIS / Production host: Cell-free synthesis (others) / References: UniProt: Q7SYL1 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 0.75 mM [U-13C; U-15N] Bis-Tris, 93% H2O/7% D2O / Solvent system: 93% H2O/7% D2O |
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Sample | Conc.: 0.75 mM / Component: Bis-Tris / Isotopic labeling: [U-13C; U-15N] |
Sample conditions | Ionic strength: 100 / pH: 6.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | |||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 20 / Conformers submitted total number: 5 |