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Yorodumi- PDB-2jzs: Solution structure of the reduced form of the N-terminal domain o... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2jzs | ||||||
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Title | Solution structure of the reduced form of the N-terminal domain of PilB from N. meningitidis. | ||||||
Components | Peptide methionine sulfoxide reductase msrA/msrB | ||||||
Keywords | ELECTRON TRANSPORT / reduced / Neisseria meningitidis / PilB / N-terminal domain / Thioredoxin / Multifunctional enzyme / Oxidoreductase / Redox-active center / Transport | ||||||
Function / homology | Function and homology information L-methionine:thioredoxin-disulfide S-oxidoreductase activity / peptide-methionine (R)-S-oxide reductase / peptide-methionine (R)-S-oxide reductase activity / peptide-methionine (S)-S-oxide reductase / peptide-methionine (S)-S-oxide reductase activity / protein repair / protein modification process / response to oxidative stress / cytoplasm Similarity search - Function | ||||||
Biological species | Neisseria meningitidis serogroup A (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Quinternet, M. / Tsan, P. / Neiers, F. / Beaufils, C. / Boschi-Muller, S. / Averlant-Petit, M. / Branlant, G. / Cung, M. | ||||||
Citation | Journal: Biochemistry / Year: 2008 Title: Solution structure and dynamics of the reduced and oxidized forms of the N-terminal domain of PilB from Neisseria meningitidis. Authors: Quinternet, M. / Tsan, P. / Neiers, F. / Beaufils, C. / Boschi-Muller, S. / Averlant-Petit, M.C. / Branlant, G. / Cung, M.T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2jzs.cif.gz | 865.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2jzs.ent.gz | 724.9 KB | Display | PDB format |
PDBx/mmJSON format | 2jzs.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jz/2jzs ftp://data.pdbj.org/pub/pdb/validation_reports/jz/2jzs | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 15848.090 Da / Num. of mol.: 1 / Fragment: Thioredoxin domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Neisseria meningitidis serogroup A (bacteria) Gene: msrAB, pilB / Production host: Escherichia coli (E. coli) / References: UniProt: Q9JWM8 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1 mM [U-100% 13C; U-100% 15N] NterPilBred, 50 mM DTT, 10 mM potassium chloride, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 10 / pH: 7 / Pressure: ambient / Temperature: 299 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 1000 / Conformers submitted total number: 20 |