+Open data
-Basic information
Entry | Database: PDB / ID: 2jul | ||||||
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Title | NMR Structure of DREAM | ||||||
Components | Calsenilin | ||||||
Keywords | DNA BINDING PROTEIN / EF-hand / calcium / LxxLL / dimer / Alternative splicing / Apoptosis / Cytoplasm / Endoplasmic reticulum / Golgi apparatus / Ion transport / Ionic channel / Lipoprotein / Membrane / Nucleus / Palmitate / Polymorphism / Potassium / Potassium channel / Potassium transport / Repressor / Transcription / Transcription regulation / Transport / Voltage-gated channel | ||||||
Function / homology | Function and homology information Phase 1 - inactivation of fast Na+ channels / : / voltage-gated monoatomic ion channel activity / regulation of potassium ion transmembrane transport / response to pain / behavioral response to pain / potassium channel activity / response to magnesium ion / potassium channel regulator activity / axon terminus ...Phase 1 - inactivation of fast Na+ channels / : / voltage-gated monoatomic ion channel activity / regulation of potassium ion transmembrane transport / response to pain / behavioral response to pain / potassium channel activity / response to magnesium ion / potassium channel regulator activity / axon terminus / regulation of neuron apoptotic process / core promoter sequence-specific DNA binding / voltage-gated potassium channel complex / sensory perception of pain / protein localization to plasma membrane / protein-DNA complex / intracellular protein transport / potassium ion transport / DNA-binding transcription repressor activity, RNA polymerase II-specific / calcium-dependent protein binding / transmembrane transporter binding / sequence-specific DNA binding / RNA polymerase II cis-regulatory region sequence-specific DNA binding / axon / negative regulation of DNA-templated transcription / apoptotic process / dendrite / calcium ion binding / Golgi apparatus / negative regulation of transcription by RNA polymerase II / magnesium ion binding / endoplasmic reticulum / DNA binding / nucleus / cytosol Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Ames, J. | ||||||
Citation | Journal: Biochemistry / Year: 2008 Title: NMR structure of DREAM: Implications for Ca(2+)-dependent DNA binding and protein dimerization. Authors: Lusin, J.D. / Vanarotti, M. / Li, C. / Valiveti, A. / Ames, J.B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2jul.cif.gz | 865.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2jul.ent.gz | 718.9 KB | Display | PDB format |
PDBx/mmJSON format | 2jul.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ju/2jul ftp://data.pdbj.org/pub/pdb/validation_reports/ju/2jul | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 29499.555 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Kcnip3, Csen, Dream, Kchip3 / Plasmid: pET23a / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q9QXT8 |
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#2: Chemical |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 0.5 mM [U-13C; U-15N] protein, 90% H2O/10% D2O / Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 0.5 mM / Component: entity_1 / Isotopic labeling: [U-13C; U-15N] |
Sample conditions | Ionic strength: 20 mM / pH: 7.4 / Pressure: ambient / Temperature: 310 K |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 15 |