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Yorodumi- PDB-2jql: NMR structure of the yeast Dun1 FHA domain in complex with a doub... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2jql | ||||||
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Title | NMR structure of the yeast Dun1 FHA domain in complex with a doubly phosphorylated (pT) peptide derived from Rad53 SCD1 | ||||||
Components |
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Keywords | CELL CYCLE / protein/phosphopeptide | ||||||
Function / homology | Function and homology information deoxyribonucleoside triphosphate biosynthetic process / G2/M DNA damage checkpoint / Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex / Ubiquitin Mediated Degradation of Phosphorylated Cdc25A / mitotic DNA damage checkpoint signaling / meiotic recombination checkpoint signaling / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / negative regulation of phosphorylation / dual-specificity kinase / DNA replication origin binding ...deoxyribonucleoside triphosphate biosynthetic process / G2/M DNA damage checkpoint / Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex / Ubiquitin Mediated Degradation of Phosphorylated Cdc25A / mitotic DNA damage checkpoint signaling / meiotic recombination checkpoint signaling / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / negative regulation of phosphorylation / dual-specificity kinase / DNA replication origin binding / negative regulation of DNA damage checkpoint / DNA replication initiation / regulation of DNA repair / protein serine/threonine/tyrosine kinase activity / DNA damage checkpoint signaling / protein localization / protein tyrosine kinase activity / non-specific serine/threonine protein kinase / protein kinase activity / phosphorylation / protein serine kinase activity / DNA repair / protein serine/threonine kinase activity / ATP binding / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Yuan, C. / Lee, H. / Chang, C. / Heierhorst, J. / Tsai, M. | ||||||
Citation | Journal: Mol.Cell / Year: 2008 Title: Diphosphothreonine-specific interaction between an SQ/TQ cluster and an FHA domain in the Rad53-Dun1 kinase cascade. Authors: Lee, H. / Yuan, C. / Hammet, A. / Mahajan, A. / Chen, E.S. / Wu, M.R. / Su, M.I. / Heierhorst, J. / Tsai, M.D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2jql.cif.gz | 946.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2jql.ent.gz | 790.6 KB | Display | PDB format |
PDBx/mmJSON format | 2jql.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jq/2jql ftp://data.pdbj.org/pub/pdb/validation_reports/jq/2jql | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 17394.494 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: DUN1 / Plasmid: pQE-60 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) References: UniProt: P39009, non-specific serine/threonine protein kinase |
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#2: Protein/peptide | Mass: 1277.126 Da / Num. of mol.: 1 / Source method: obtained synthetically References: UniProt: P22216, non-specific serine/threonine protein kinase |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 150 mM NaCl / pH: 7.5 / Pressure: ambient / Temperature: 293 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||||||||
NMR representative | Selection criteria: closest to the average | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 20 |