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Open data
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Basic information
Entry | Database: PDB / ID: 2j3r | ||||||
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Title | The crystal structure of the bet3-trs31 heterodimer. | ||||||
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![]() | TRANSPORT / ![]() ![]() ![]() ![]() ![]() ![]() | ||||||
Function / homology | ![]() COPII-mediated vesicle transport / RAB GEFs exchange GTP for GDP on RABs / vesicle coating / vesicle tethering / RAB GEFs exchange GTP for GDP on RABs / TRAPPI protein complex / COPII-mediated vesicle transport / TRAPPII protein complex / TRAPPIII protein complex / ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kim, Y.-G. / Oh, B.-H. | ||||||
![]() | ![]() Title: The Architecture of the Multisubunit Trapp I Complex Suggests a Model for Vesicle Tethering. Authors: Kim, Y.-G. / Raunser, S. / Munger, C. / Wagner, J. / Song, Y.-L. / Cygler, M. / Walz, T. / Oh, B.-H. / Sacher, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 70.5 KB | Display | ![]() |
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PDB format | ![]() | 56.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 20452.227 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() |
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#2: Protein | Mass: 17536.230 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() |
#3: Chemical | ChemComp-PLM / ![]() |
#4: Chemical | ChemComp-NO3 / ![]() |
#5: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.08 Å3/Da / Density % sol: 60.13 % |
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Crystal grow![]() | pH: 8 Details: 0.26 M AMMONIUM NITRATE AND 14% (W/V) POLYETHYLENE GLYCOL MONOMETHYL ETHER 5000, pH 8.00 |
-Data collection
Diffraction | Mean temperature: 113 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.56→30 Å / Num. obs: 14269 / % possible obs: 93.2 % / Observed criterion σ(I): 0 / Redundancy: 5.7 % / Rmerge(I) obs: 0.04 / Net I/σ(I): 53.2 |
Reflection shell | Highest resolution: 2.56 Å / Rmerge(I) obs: 0.11 / Mean I/σ(I) obs: 10.5 / % possible all: 78.5 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Bsol: 32.4956 Å2 / ksol: 0.310941 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.6→30 Å
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Refine LS restraints |
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Xplor file |
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