+Open data
-Basic information
Entry | Database: PDB / ID: 2iic | ||||||
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Title | Calcium bound structure of alpha-11 giardin | ||||||
Components | Alpha-11 giardin | ||||||
Keywords | METAL BINDING PROTEIN / calcium-protein complex / helix-turn-helix | ||||||
Function / homology | Function and homology information calcium-dependent phospholipid binding / cytoskeleton organization / microtubule / calcium ion binding Similarity search - Function | ||||||
Biological species | Giardia intestinalis (eukaryote) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.93 Å | ||||||
Authors | Pathuri, P. / Nguyen, E.T. / Luecke, H. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2007 Title: Apo and Calcium-bound Crystal Structures of Alpha-11 Giardin, an Unusual Annexin from Giardia lamblia Authors: Pathuri, P. / Nguyen, E.T. / Svard, S.G. / Luecke, H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2iic.cif.gz | 246.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2iic.ent.gz | 198.8 KB | Display | PDB format |
PDBx/mmJSON format | 2iic.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ii/2iic ftp://data.pdbj.org/pub/pdb/validation_reports/ii/2iic | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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Unit cell |
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-Components
#1: Protein | Mass: 35252.930 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Giardia intestinalis (eukaryote) / Plasmid: pGEX-6P3 / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta (DE3) / References: UniProt: Q4VPP2 #2: Chemical | ChemComp-CA / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.74 Å3/Da / Density % sol: 50.2 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: 100mM Bis-Tris pH 6.5, 24% PEG 550 MME, 50mM calcium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
-Data collection
Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL9-1 / Wavelength: 0.979 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jul 19, 2006 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Redundancy: 3.7 % / Av σ(I) over netI: 6.8 / Number: 112381 / Rmerge(I) obs: 0.104 / Χ2: 1.12 / D res high: 2.91 Å / D res low: 50 Å / Num. obs: 30731 / % possible obs: 99.6 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Diffraction reflection shell |
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Reflection | Resolution: 2.91→50 Å / Num. obs: 30731 / % possible obs: 99.6 % / Redundancy: 3.7 % / Rmerge(I) obs: 0.104 / Χ2: 1.117 / Net I/σ(I): 6.8 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Resolution: 2.91→3.01 Å / Redundancy: 3.6 % / Rmerge(I) obs: 0.493 / Num. unique all: 3054 / Χ2: 0.99 / % possible all: 99.4 |
-Phasing
Phasing MR |
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.93→40 Å / σ(F): 0
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Solvent computation | Bsol: 29.473 Å2 | ||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 49.595 Å2 | ||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.93→40 Å
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Refine LS restraints |
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Xplor file |
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