+Open data
-Basic information
Entry | Database: PDB / ID: 2hgl | ||||||
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Title | NMR structure of the first qRRM domain of human hnRNP F | ||||||
Components | Heterogeneous nuclear ribonucleoprotein F | ||||||
Keywords | RNA BINDING PROTEIN / RNA recognition Motif / G-tract / G-quadruplex / alternative / splicing | ||||||
Function / homology | Function and homology information FGFR2 alternative splicing / regulation of RNA splicing / Processing of Capped Intron-Containing Pre-mRNA / RNA processing / Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation / catalytic step 2 spliceosome / mRNA Splicing - Major Pathway / mRNA splicing, via spliceosome / single-stranded RNA binding / ribonucleoprotein complex ...FGFR2 alternative splicing / regulation of RNA splicing / Processing of Capped Intron-Containing Pre-mRNA / RNA processing / Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation / catalytic step 2 spliceosome / mRNA Splicing - Major Pathway / mRNA splicing, via spliceosome / single-stranded RNA binding / ribonucleoprotein complex / synapse / RNA binding / nucleoplasm / membrane / nucleus / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Dominguez, C. / Allain, F.H.-T. | ||||||
Citation | Journal: Nucleic Acids Res. / Year: 2006 Title: NMR structure of the three quasi RNA recognition motifs (qRRMs) of human hnRNP F and interaction studies with Bcl-x G-tract RNA: a novel mode of RNA recognition. Authors: Dominguez, C. / Allain, F.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2hgl.cif.gz | 468.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2hgl.ent.gz | 385.3 KB | Display | PDB format |
PDBx/mmJSON format | 2hgl.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hg/2hgl ftp://data.pdbj.org/pub/pdb/validation_reports/hg/2hgl | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 15027.921 Da / Num. of mol.: 1 / Fragment: quasi RNA recognition Motif 1 (qRRM1) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: HNRPF / Plasmid: Pet28b(+) / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) Codon plus / References: UniProt: Q5T0N2, UniProt: P52597*PLUS |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample conditions | Ionic strength: 25mM NaH2PO4, 50 mM NaCl / pH: 6.2 / Pressure: ambient / Temperature: 313 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | ||||||||||||||||||||
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Radiation wavelength | Relative weight: 1 | ||||||||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 15 |