[English] 日本語
Yorodumi- PDB-2gzz: solution structures of the oxidized form of thioredoxin from Baci... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2gzz | ||||||
---|---|---|---|---|---|---|---|
Title | solution structures of the oxidized form of thioredoxin from Bacillus subtilis | ||||||
Components | thioredoxin | ||||||
Keywords | ELECTRON TRANSPORT / alpha/beta | ||||||
Function / homology | Function and homology information protein-disulfide reductase activity / cell redox homeostasis / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Bacillus subtilis (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Xu, H. / Zhang, X. / Chen, J. / Jin, C. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2007 Title: Conformational fluctuations coupled to the thiol-disulfide transfer between thioredoxin and arsenate reductase in Bacillus subtilis. Authors: Li, Y. / Hu, Y. / Zhang, X. / Xu, H. / Lescop, E. / Xia, B. / Jin, C. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2gzz.cif.gz | 617.8 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2gzz.ent.gz | 539.3 KB | Display | PDB format |
PDBx/mmJSON format | 2gzz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gz/2gzz ftp://data.pdbj.org/pub/pdb/validation_reports/gz/2gzz | HTTPS FTP |
---|
-Related structure data
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 11404.136 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus subtilis (bacteria) / Plasmid: pET21a / Production host: Escherichia coli (E. coli) / References: UniProt: P14949 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
| ||||||||||||
NMR details | Text: The structure was determined using triple-resonance NMR spectroscopy. |
-Sample preparation
Details | Contents: 1mM thioredoxin U-15N, 13C; 20mM Tris-HCl buffer / Solvent system: 90% H2O/10% D2O |
---|---|
Sample conditions | Ionic strength: 20mM Tris-HCl / pH: 6.8 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz |
---|
-Processing
NMR software | Name: Amber / Version: 7 / Developer: Case, D.A. et al. / Classification: refinement |
---|---|
Refinement | Method: simulated annealing / Software ordinal: 1 |
NMR representative | Selection criteria: fewest violations, lowest energy |
NMR ensemble | Conformer selection criteria: structures with the least restraint violations, structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |