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Yorodumi- PDB-2gsq: GLUTATHIONE S-TRANSFERASE FROM SQUID DIGESTIVE GLAND COMPLEXED WI... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2gsq | ||||||
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Title | GLUTATHIONE S-TRANSFERASE FROM SQUID DIGESTIVE GLAND COMPLEXED WITH S-(3-IODOBENZYL)GLUTATHIONE | ||||||
Components | GLUTATHIONE S-TRANSFERASE | ||||||
Keywords | TRANSFERASE / SQUID DIGESTIVE GLAND / SIGMA CLASS | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Ommastrephes sloani (Sloane's squid) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.2 Å | ||||||
Authors | Ji, X. / Armstrong, R.N. / Gilliland, G.L. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 1996 Title: Location of a potential transport binding site in a sigma class glutathione transferase by x-ray crystallography. Authors: Ji, X. / von Rosenvinge, E.C. / Johnson, W.W. / Armstrong, R.N. / Gilliland, G.L. #1: Journal: Biochemistry / Year: 1995 Title: Three-Dimensional Structure, Catalytic Properties and Evolution of a Sigma Class Glutathione Transferase from Squid, a Progenitor of the Lens-Crystallins of Cephalopods Authors: Ji, X. / Von Rosenvinge, E.C. / Johnson, W.W. / Tomarev, S.I. / Piatigorsky, J. / Armstrong, R.N. / Gilliland, G.L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2gsq.cif.gz | 60 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2gsq.ent.gz | 43.2 KB | Display | PDB format |
PDBx/mmJSON format | 2gsq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gs/2gsq ftp://data.pdbj.org/pub/pdb/validation_reports/gs/2gsq | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO 51 | ||||||||
Components on special symmetry positions |
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-Components
#1: Protein | Mass: 22820.578 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Ommastrephes sloani (Sloane's squid) / Gene: CDNA INSERT OF CLONE PGST5 / Organ: DIGESTIVE GLAND / Plasmid: GST5/PET / Gene (production host): CDNA INSERT OF CLONE PGST5 / Production host: Escherichia coli (E. coli) / Strain (production host): DH5A / References: UniProt: P46088, glutathione transferase | ||
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#2: Chemical | ChemComp-SO4 / | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 3.21 Å3/Da / Density % sol: 61.68 % | |||||||||||||||||||||||||||||||||||
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Crystal | *PLUS Density % sol: 55 % | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / pH: 7 / Method: vapor diffusion, sitting drop | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Detector | Type: ELECTRONICS COMPUTING TECHNOLOGIES / Detector: AREA DETECTOR / Date: May 29, 1994 |
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Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Num. obs: 15331 / % possible obs: 99.7 % / Observed criterion σ(I): 0 / Redundancy: 3.1 % / Rmerge(I) obs: 0.09 |
Reflection | *PLUS Highest resolution: 2.2 Å / Rmerge(I) obs: 0.09 |
Reflection shell | *PLUS Highest resolution: 2.2 Å / Lowest resolution: 2.34 Å / Mean I/σ(I) obs: 1.9 |
-Processing
Software |
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Refinement | Resolution: 2.2→6 Å / σ(F): 2 /
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Displacement parameters | Biso mean: 23.12 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→6 Å
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Refine LS restraints |
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Software | *PLUS Name: PROLSQ / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS σ(I): 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |