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Yorodumi- PDB-2gqc: Solution structure of the N-terminal domain of Rhomboid Intramemb... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2gqc | ||||||
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Title | Solution structure of the N-terminal domain of Rhomboid Intramembrane Protease from P. aeruginosa | ||||||
Components | Rhomboid Intramembrane Protease | ||||||
Keywords | HYDROLASE / alpha-beta domain | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Pseudomonas aeruginosa (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing torsional angle dynamics | ||||||
Authors | Dutta, K. / Del Rio, A. / Chavez, J. / Ubarretxena-Belandia, I. / Ghose, R. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2007 Title: Solution structure and dynamics of the N-terminal cytosolic domain of rhomboid intramembrane protease from Pseudomonas aeruginosa: insights into a functional role in intramembrane proteolysis. Authors: Del Rio, A. / Dutta, K. / Chavez, J. / Ubarretxena-Belandia, I. / Ghose, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2gqc.cif.gz | 434.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2gqc.ent.gz | 360.6 KB | Display | PDB format |
PDBx/mmJSON format | 2gqc.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gq/2gqc ftp://data.pdbj.org/pub/pdb/validation_reports/gq/2gqc | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 7941.021 Da / Num. of mol.: 1 / Fragment: N-terminal Domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) / Strain: PAO1 / Plasmid: pET 15b / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 References: GenBank: 9949195, UniProt: Q9HZC2*PLUS, rhomboid protease |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D and 3D heteronuclear techniques. |
-Sample preparation
Details | Contents: 1 mM, U-15N,13C; 20 mM Bis-Tris, 50 mM NaCl pH 6.5 Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 50 mM NaCl / pH: 6.5 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||
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Radiation wavelength | Relative weight: 1 | ||||||||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing torsional angle dynamics / Software ordinal: 1 | |||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 1500 / Conformers submitted total number: 20 |