+Open data
-Basic information
Entry | Database: PDB / ID: 2goo | |||||||||
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Title | Ternary Complex of BMP-2 bound to BMPR-Ia-ECD and ActRII-ECD | |||||||||
Components |
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Keywords | TRANSFERASE / TGF-beta / BMP-2 / BMPR-Ia / ActRII / ALK-3 | |||||||||
Function / homology | Function and homology information Signaling by Activin / neural plate mediolateral regionalization / paraxial mesoderm structural organization / positive regulation of cardiac ventricle development / fibrous ring of heart morphogenesis / inhibin-betaglycan-ActRII complex / positive regulation of transforming growth factor beta2 production / inhibin binding / Mullerian duct regression / Signaling by BMP ...Signaling by Activin / neural plate mediolateral regionalization / paraxial mesoderm structural organization / positive regulation of cardiac ventricle development / fibrous ring of heart morphogenesis / inhibin-betaglycan-ActRII complex / positive regulation of transforming growth factor beta2 production / inhibin binding / Mullerian duct regression / Signaling by BMP / cardiac atrium formation / cardiocyte differentiation / negative regulation of calcium-independent cell-cell adhesion / cardiac jelly development / heart formation / atrioventricular node cell development / negative regulation of aldosterone biosynthetic process / embryonic heart tube anterior/posterior pattern specification / atrioventricular canal morphogenesis / negative regulation of cortisol biosynthetic process / penile erection / mesenchymal cell proliferation involved in ureteric bud development / mesendoderm development / negative regulation of steroid biosynthetic process / ameloblast differentiation / tricuspid valve morphogenesis / positive regulation of activin receptor signaling pathway / positive regulation of extracellular matrix constituent secretion / atrioventricular valve development / activin receptor activity / dorsal aorta morphogenesis / negative regulation of cardiac muscle cell differentiation / regulation of odontogenesis of dentin-containing tooth / endodermal-mesodermal cell signaling / corticotropin hormone secreting cell differentiation / central nervous system neuron differentiation / cardiac right ventricle morphogenesis / negative regulation of insulin-like growth factor receptor signaling pathway / thyroid-stimulating hormone-secreting cell differentiation / mesenchyme development / pharyngeal arch artery morphogenesis / aortic valve development / BMP binding / telencephalon regionalization / positive regulation of follicle-stimulating hormone secretion / hindlimb morphogenesis / cellular response to oxygen-glucose deprivation / positive regulation of phosphatase activity / positive regulation of odontogenesis / regulation of cardiac muscle cell proliferation / negative regulation of muscle cell differentiation / Sertoli cell proliferation / negative regulation of smooth muscle cell migration / positive regulation of cartilage development / proteoglycan metabolic process / heart induction / positive regulation of peroxisome proliferator activated receptor signaling pathway / sperm ejaculation / lateral mesoderm development / regulation of lateral mesodermal cell fate specification / ventricular compact myocardium morphogenesis / pericardium development / pituitary gland development / lung vasculature development / mitral valve morphogenesis / BMP receptor complex / BMP receptor activity / dorsal/ventral axis specification / embryonic skeletal system development / co-receptor binding / regulation of cellular senescence / neural crest cell development / telencephalon development / cardiac epithelial to mesenchymal transition / transforming growth factor beta receptor activity, type I / activin receptor complex / mesenchymal cell differentiation / BMP receptor binding / ectoderm development / cardiac conduction system development / positive regulation of odontoblast differentiation / endocardial cushion formation / positive regulation of bone mineralization involved in bone maturation / phosphatase activator activity / Transcriptional regulation by RUNX2 / receptor protein serine/threonine kinase / positive regulation of astrocyte differentiation / transmembrane receptor protein serine/threonine kinase activity / Signaling by BMP / activin binding / cellular response to BMP stimulus / cardiac muscle cell differentiation / activin receptor signaling pathway / outflow tract septum morphogenesis / cardiac muscle tissue morphogenesis / ventricular trabecula myocardium morphogenesis / SMAD protein signal transduction / positive regulation of ossification / astrocyte differentiation / gastrulation with mouth forming second Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) Mus musculus (house mouse) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.2 Å | |||||||||
Authors | Allendorph, G.P. / Choe, S. | |||||||||
Citation | Journal: Proc.Natl.Acad.Sci.Usa / Year: 2006 Title: Structure of the ternary signaling complex of a TGF-beta superfamily member. Authors: Allendorph, G.P. / Vale, W.W. / Choe, S. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2goo.cif.gz | 129.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2goo.ent.gz | 104.7 KB | Display | PDB format |
PDBx/mmJSON format | 2goo.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/go/2goo ftp://data.pdbj.org/pub/pdb/validation_reports/go/2goo | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | The asymmetric unit consists of two subunits, each subunit is one half of the biological dimer. The second half of the dimer is generated by the 2-fold axis: y, x, (z-1) + 2/3 |
-Components
#1: Protein | Mass: 12923.854 Da / Num. of mol.: 2 / Fragment: Residues 283-396 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: BMP2 / Production host: Escherichia coli (E. coli) / References: UniProt: P12643 #2: Protein | Mass: 14344.048 Da / Num. of mol.: 2 / Fragment: Residues 24-152 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: BMPR1A / Production host: Escherichia coli (E. coli) References: UniProt: P36894, receptor protein serine/threonine kinase #3: Protein | Mass: 12019.440 Da / Num. of mol.: 2 / Fragment: Residues 20-121 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Acvr2a / Production host: Pichia pastoris (fungus) References: UniProt: P27038, receptor protein serine/threonine kinase #4: Sugar | ChemComp-NDG / #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 2 |
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-Sample preparation
Crystal | Density Matthews: 3.6 Å3/Da / Density % sol: 65.85 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 4M sodium formate, 100mM Hepes, 3% dioxane, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
-Data collection
Diffraction |
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Diffraction source |
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Detector |
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Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 | |||||||||||||||
Reflection | Resolution: 2.15→50 Å / Num. all: 64371 / Num. obs: 62054 / % possible obs: 96.4 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 7.3 % / Rmerge(I) obs: 0.085 / Χ2: 1.062 / Net I/σ(I): 15.5 | |||||||||||||||
Reflection shell | Resolution: 2.15→2.23 Å / % possible obs: 89.4 % / Redundancy: 5.9 % / Rmerge(I) obs: 0.402 / Num. unique obs: 5626 / Χ2: 0.989 / % possible all: 89.4 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.2→50 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 41.247 Å2 | ||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→50 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.2→2.257 Å
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