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Yorodumi- PDB-2fv4: NMR solution structure of the yeast kinetochore Spc24/Spc25 globu... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2fv4 | ||||||
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Title | NMR solution structure of the yeast kinetochore Spc24/Spc25 globular domain | ||||||
Components |
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Keywords | STRUCTURAL PROTEIN / PROTEIN BINDING / alpha-beta / complex / coiled-coil | ||||||
Function / homology | Function and homology information centromere clustering / Ndc80 complex / sister chromatid biorientation / chromosome segregation / kinetochore / cell division / identical protein binding / nucleus Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Schnell, J.R. / Chou, J.J. | ||||||
Citation | Journal: Structure / Year: 2006 Title: Structure of a central component of the yeast kinetochore: the spc24p/spc25p globular domain. Authors: Wei, R.R. / Schnell, J.R. / Larsen, N.A. / Sorger, P.K. / Chou, J.J. / Harrison, S.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2fv4.cif.gz | 915.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2fv4.ent.gz | 776.4 KB | Display | PDB format |
PDBx/mmJSON format | 2fv4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fv/2fv4 ftp://data.pdbj.org/pub/pdb/validation_reports/fv/2fv4 | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10585.985 Da / Num. of mol.: 1 / Fragment: SPC25P GLOBULAR DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: YER018C / Plasmid: PET3ATR / Cell line (production host): ROSETTA / Production host: Escherichia coli (E. coli) / References: UniProt: P40014 |
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#2: Protein | Mass: 8926.170 Da / Num. of mol.: 1 / Fragment: SPC24P GLOBULAR DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: YMR117C, YM9718.16C / Plasmid: PET3ATR / Cell line (production host): ROSETTA / Production host: Escherichia coli (E. coli) / References: UniProt: Q04477 |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample conditions | Ionic strength: 50 mM sodium phosphate buffer / pH: 7.0 / Pressure: ambient / Temperature: 303 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz |
-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 30 / Conformers submitted total number: 20 |