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Open data
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Basic information
Entry | Database: PDB / ID: 2fua | ||||||
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Title | L-FUCULOSE 1-PHOSPHATE ALDOLASE CRYSTAL FORM T WITH COBALT | ||||||
![]() | L-FUCULOSE-1-PHOSPHATE ALDOLASE | ||||||
![]() | LYASE (ALDEHYDE) / CLASS II ALDOLASE | ||||||
Function / homology | ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Dreyer, M.K. / Schulz, G.E. | ||||||
![]() | ![]() Title: Refined high-resolution structure of the metal-ion dependent L-fuculose-1-phosphate aldolase (class II) from Escherichia coli. Authors: Dreyer, M.K. / Schulz, G.E. #1: ![]() Title: Catalytic Mechanism of the Metal-Dependent Fuculose Aldolase from Escherichia Coli as Derived from the Structure Authors: Dreyer, M.K. / Schulz, G.E. #2: ![]() Title: The Spatial Structure of the Class II L-Fuculose-1-Phosphate Aldolase from Escherichia Coli Authors: Dreyer, M.K. / Schulz, G.E. #3: ![]() Title: Diastereoselective Enzymatic Aldol Additions: L-Rhamnulose and L-Fuculose 1-Phosphate Aldolases from E.Coli Authors: Fessner, W.-D. / Sinerius, G. / Schneider, A. / Dreyer, M. / Schulz, G.E. / Badia, J. / Aguilar, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 56.6 KB | Display | ![]() |
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PDB format | ![]() | 41.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 23805.318 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: COBALT IN THIS STRUCTURE REPLACES THE NATURALLY OCCURRING ZINC ION Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() ![]() | ||||
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#2: Chemical | ChemComp-CO / | ||||
#3: Chemical | ![]() #4: Chemical | ChemComp-BME / | ![]() #5: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.97 Å3/Da / Density % sol: 38 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow![]() | *PLUS Temperature: 293 K / pH: 7.6 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: SIEMENS-NICOLET X100 / Detector: AREA DETECTOR / Date: 1994 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Highest resolution: 1.8 Å / Num. obs: 15207 / Observed criterion σ(I): 0 / Redundancy: 2.7 % / Rmerge(I) obs: 0.061 |
Reflection | *PLUS Highest resolution: 2 Å / Lowest resolution: 10 Å / % possible obs: 89 % |
Reflection shell | *PLUS Highest resolution: 2 Å / Lowest resolution: 2.04 Å / % possible obs: 80 % / Rmerge(I) obs: 0.159 |
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Processing
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Refinement | Resolution: 2→10 Å / σ(F): 0 Details: LOOP 23 - 27, WHICH IS NEAR THE ACTIVE SITE AND PARTICIPATES IN THE SUBUNIT INTERFACE, IS MOBILE, HAS ONLY POOR DENSITY AND THE COORDINATES ARE NOT RELIABLE. IN COMPARISON WITH THE ZINC- ...Details: LOOP 23 - 27, WHICH IS NEAR THE ACTIVE SITE AND PARTICIPATES IN THE SUBUNIT INTERFACE, IS MOBILE, HAS ONLY POOR DENSITY AND THE COORDINATES ARE NOT RELIABLE. IN COMPARISON WITH THE ZINC-CONTAINING STRUCTURE, ADDITIONAL DENSITY APPEARED FOR RESIDUES 207 - 210; STILL, THE FIVE C-TERMINAL RESIDUES 211 - 215 CANNOT BE LOCATED IN THE ELECTRON DENSITY MAP. THE OCCUPANCY OF SULFATE ION 301 WAS REFINED TO 0.74.
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Displacement parameters | Biso mean: 18.4 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.23 Å / Luzzati sigma a obs: 0.22 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→10 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.18 / Rfactor Rwork![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |