+Open data
-Basic information
Entry | Database: PDB / ID: 2eyw | ||||||
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Title | N-terminal SH3 domain of CT10-Regulated Kinase | ||||||
Components | v-crk sarcoma virus CT10 oncogene homolog isoform a | ||||||
Keywords | SIGNALING PROTEIN / SH3 | ||||||
Function / homology | Function and homology information response to hepatocyte growth factor / regulation of intracellular signal transduction / cellular response to endothelin / helper T cell diapedesis / cerebellar neuron development / response to cholecystokinin / postsynaptic specialization assembly / protein phosphorylated amino acid binding / regulation of T cell migration / reelin-mediated signaling pathway ...response to hepatocyte growth factor / regulation of intracellular signal transduction / cellular response to endothelin / helper T cell diapedesis / cerebellar neuron development / response to cholecystokinin / postsynaptic specialization assembly / protein phosphorylated amino acid binding / regulation of T cell migration / reelin-mediated signaling pathway / regulation of dendrite development / positive regulation of skeletal muscle acetylcholine-gated channel clustering / response to yeast / negative regulation of wound healing / negative regulation of natural killer cell mediated cytotoxicity / regulation of protein binding / negative regulation of cell motility / ARMS-mediated activation / MET receptor recycling / protein localization to membrane / MET activates RAP1 and RAC1 / cellular response to insulin-like growth factor stimulus / establishment of cell polarity / regulation of GTPase activity / p130Cas linkage to MAPK signaling for integrins / positive regulation of smooth muscle cell migration / dendrite development / positive regulation of Rac protein signal transduction / regulation of cell adhesion mediated by integrin / cellular response to nitric oxide / regulation of signal transduction / ephrin receptor signaling pathway / PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases / signaling adaptor activity / positive regulation of substrate adhesion-dependent cell spreading / cellular response to transforming growth factor beta stimulus / insulin-like growth factor receptor binding / cytoskeletal protein binding / phosphotyrosine residue binding / SH2 domain binding / ephrin receptor binding / cell chemotaxis / Downstream signal transduction / protein tyrosine kinase binding / cellular response to nerve growth factor stimulus / Regulation of signaling by CBL / hippocampus development / regulation of actin cytoskeleton organization / FCGR3A-mediated phagocytosis / positive regulation of JNK cascade / neuron migration / neuromuscular junction / response to hydrogen peroxide / lipid metabolic process / receptor tyrosine kinase binding / Regulation of actin dynamics for phagocytic cup formation / cerebral cortex development / kinase binding / VEGFA-VEGFR2 Pathway / SH3 domain binding / : / signaling receptor complex adaptor activity / cell migration / actin cytoskeleton / regulation of cell shape / actin cytoskeleton organization / scaffold protein binding / positive regulation of cell growth / cell population proliferation / ubiquitin protein ligase binding / regulation of transcription by RNA polymerase II / signal transduction / protein-containing complex / extracellular exosome / membrane / nucleus / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Kobashigawa, Y. / Tanaka, S. / Inagaki, F. | ||||||
Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2007 Title: Structural basis for the transforming activity of human cancer-related signaling adaptor protein CRK. Authors: Kobashigawa, Y. / Sakai, M. / Naito, M. / Yokochi, M. / Kumeta, H. / Makino, Y. / Ogura, K. / Tanaka, S. / Inagaki, F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2eyw.cif.gz | 37.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2eyw.ent.gz | 26.8 KB | Display | PDB format |
PDBx/mmJSON format | 2eyw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ey/2eyw ftp://data.pdbj.org/pub/pdb/validation_reports/ey/2eyw | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 8900.870 Da / Num. of mol.: 1 / Fragment: N-terminal SH3 domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Plasmid: pPRO EX-htb / Production host: Escherichia coli (E. coli) / References: UniProt: P46108 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 0.5mM N-terminal SH3 U-15N, 13C PHOSPHATE BUFFER NA; 200MM NACL;90%H2O, 10%D2O |
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Sample conditions | Ionic strength: 250mM / pH: 6.8 / Pressure: AMBIENT / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 800 MHz |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformers calculated total number: 20 / Conformers submitted total number: 1 |