+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 2eqe | ||||||
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タイトル | Solution structure of the fourth A20-type zinc finger domain from human tumor necrosis factor, alpha-induced protein3 | ||||||
要素 | Tumor necrosis factor, alpha-induced protein 3 | ||||||
キーワード | HYDROLASE (加水分解酵素) / zf-A20 domain / Tumor necrosis factor / alpha-induced protein 3 / Putative DNA-binding protein A20 / Zinc finger protein A20 (ジンクフィンガー) / Structural Genomics (構造ゲノミクス) / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
機能・相同性 | 機能・相同性情報 regulation of vascular wound healing / negative regulation of toll-like receptor 5 signaling pathway / negative regulation of nucleotide-binding oligomerization domain containing 1 signaling pathway / establishment of protein localization to vacuole / negative regulation of osteoclast proliferation / tolerance induction to lipopolysaccharide / negative regulation of CD40 signaling pathway / negative regulation of toll-like receptor 3 signaling pathway / negative regulation of B cell activation / negative regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway ...regulation of vascular wound healing / negative regulation of toll-like receptor 5 signaling pathway / negative regulation of nucleotide-binding oligomerization domain containing 1 signaling pathway / establishment of protein localization to vacuole / negative regulation of osteoclast proliferation / tolerance induction to lipopolysaccharide / negative regulation of CD40 signaling pathway / negative regulation of toll-like receptor 3 signaling pathway / negative regulation of B cell activation / negative regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway / negative regulation of toll-like receptor 2 signaling pathway / protein K11-linked deubiquitination / nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway / negative regulation of chronic inflammatory response / negative regulation of toll-like receptor 4 signaling pathway / regulation of germinal center formation / protein K48-linked deubiquitination / B-1 B cell homeostasis / regulation of defense response to virus by host / regulation of tumor necrosis factor-mediated signaling pathway / 転移酵素; アシル基を移すもの; アミノアシル基を移すもの / protein K63-linked deubiquitination / positive regulation of hepatocyte proliferation / negative regulation of bone resorption / TNFR1-induced proapoptotic signaling / negative regulation of interleukin-1 beta production / negative regulation of interleukin-2 production / K63-linked polyubiquitin modification-dependent protein binding / K63-linked deubiquitinase activity / negative regulation of NF-kappaB transcription factor activity / protein deubiquitination / negative regulation of interleukin-6 production / response to muramyl dipeptide / negative regulation of tumor necrosis factor production / positive regulation of Wnt signaling pathway / protein K48-linked ubiquitination / negative regulation of endothelial cell apoptotic process / negative regulation of canonical NF-kappaB signal transduction / negative regulation of extrinsic apoptotic signaling pathway via death domain receptors / negative regulation of protein ubiquitination / cytoskeleton organization / negative regulation of innate immune response / TNFR1-induced NF-kappa-B signaling pathway / ubiquitin binding / Negative regulators of DDX58/IFIH1 signaling / negative regulation of smooth muscle cell proliferation / Regulation of TNFR1 signaling / NOD1/2 Signaling Pathway / response to molecule of bacterial origin / kinase binding / negative regulation of inflammatory response / cellular response to hydrogen peroxide / positive regulation of protein catabolic process / ubiquitin-protein transferase activity / : / Ovarian tumor domain proteases / 遊走 / protease binding / cellular response to lipopolysaccharide / ubiquitinyl hydrolase 1 / cysteine-type deubiquitinase activity / リソソーム / 炎症 / apoptotic process / タンパク質分解 / DNA binding / extracellular exosome / zinc ion binding / identical protein binding / 細胞核 / 細胞質基質 / 細胞質 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | 溶液NMR / torsion angle dynamics | ||||||
データ登録者 | Zhang, H.P. / Nagasima, T. / Hayahsi, F. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
引用 | ジャーナル: To be Published タイトル: Solution structure of the fourth A20-type zinc finger domain from human tumor necrosis factor, alpha-induced protein3 著者: Zhang, H.P. / Nagasima, T. / Hayashi, F. / Yokoyama, S. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 2eqe.cif.gz | 257.1 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb2eqe.ent.gz | 213.3 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 2eqe.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/eq/2eqe ftp://data.pdbj.org/pub/pdb/validation_reports/eq/2eqe | HTTPS FTP |
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-関連構造データ
類似構造データ | |
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その他のデータベース |
-リンク
-集合体
登録構造単位 |
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NMR アンサンブル |
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-要素
#1: タンパク質・ペプチド | 分子量: 4848.327 Da / 分子数: 1 / 断片: A20-type zinc finger domain / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 解説: cell-free protein synthesis / 遺伝子: TNFAIP3 / プラスミド: P070115-06 / 参照: UniProt: P21580, 加水分解酵素 |
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#2: 化合物 | ChemComp-ZN / |
-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||
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NMR実験 |
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-試料調製
詳細 | 内容: 1.16mM 13C, 15N-labeled protein; 20mM d-Tris-HCl pH 7.0); 100mM NaCl; 1mM d-DTT; 0.02% NaN3; 50mM ZnCl2, 1mM IDA; 90% H2O, 10% D2O 溶媒系: 90% H2O/10% D2O |
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試料状態 | イオン強度: 120mM / pH: 7.0 / 圧: ambient / 温度: 293 K |
-NMR測定
NMRスペクトロメーター | タイプ: Varian INOVA / 製造業者: Varian / モデル: INOVA / 磁場強度: 800 MHz |
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-解析
NMR software |
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精密化 | 手法: torsion angle dynamics / ソフトェア番号: 1 | ||||||||||||||||||||||||||||
代表構造 | 選択基準: lowest energy | ||||||||||||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the least restraint violations, tructures with the lowest energy, arget function 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 20 |