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Yorodumi- PDB-2ctn: STRUCTURE OF CALCIUM-SATURATED CARDIAC TROPONIN C, NMR, 30 STRUCTURES -
+Open data
-Basic information
Entry | Database: PDB / ID: 2ctn | ||||||
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Title | STRUCTURE OF CALCIUM-SATURATED CARDIAC TROPONIN C, NMR, 30 STRUCTURES | ||||||
Components | TROPONIN C | ||||||
Keywords | CALCIUM-BINDING PROTEIN / CARDIAC / MUSCLE / REGULATORY | ||||||
Function / homology | Function and homology information cardiac Troponin complex / Striated Muscle Contraction / myosin II complex / troponin I binding / skeletal muscle contraction / cardiac muscle contraction / calcium-dependent protein binding / calcium ion binding Similarity search - Function | ||||||
Biological species | Gallus gallus (chicken) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Sia, S.K. / Li, M.X. / Spyracopoulos, L. / Gagne, S.M. / Liu, W. / Putkey, J.A. / Sykes, B.D. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 1997 Title: Structure of cardiac muscle troponin C unexpectedly reveals a closed regulatory domain. Authors: Sia, S.K. / Li, M.X. / Spyracopoulos, L. / Gagne, S.M. / Liu, W. / Putkey, J.A. / Sykes, B.D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2ctn.cif.gz | 867 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2ctn.ent.gz | 740 KB | Display | PDB format |
PDBx/mmJSON format | 2ctn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ct/2ctn ftp://data.pdbj.org/pub/pdb/validation_reports/ct/2ctn | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 9934.044 Da / Num. of mol.: 1 / Fragment: REGULATORY N-DOMAIN RESIDUES 2 - 89 / Mutation: D2A, C35S, C84S Source method: isolated from a genetically manipulated source Details: CARDIAC TROPONIN C WITH CALCIUM ION BOUND AT SITE II Source: (gene. exp.) Gallus gallus (chicken) / Tissue: MUSCLESkeletal muscle / Cell line: BL21 / Cellular location: THIN FILAMENTMyofilament / Gene: CTNC(A-CYS) / Organ: HEART / Plasmid: PET-23D / Gene (production host): CTNC(A-CYS) / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) PLYSS / References: UniProt: P09860 |
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#2: Chemical | ChemComp-CA / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Sample conditions | pH: 6.7 / Temperature: 303 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
Software |
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NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION. | ||||||||||||
NMR ensemble | Conformer selection criteria: LOWEST TOTAL ENERGY / Conformers calculated total number: 35 / Conformers submitted total number: 30 |