+Open data
-Basic information
Entry | Database: PDB / ID: 2bnh | |||||||||
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Title | PORCINE RIBONUCLEASE INHIBITOR | |||||||||
Components | RIBONUCLEASE INHIBITOR | |||||||||
Keywords | ACETYLATION / LEUCINE-RICH REPEATS | |||||||||
Function / homology | Function and homology information ribonuclease inhibitor activity / angiogenin-PRI complex / regulation of Arp2/3 complex-mediated actin nucleation / regulation of angiogenesis / cell migration / lamellipodium / regulation of inflammatory response / nucleoplasm / plasma membrane / cytoplasm Similarity search - Function | |||||||||
Biological species | Sus scrofa (pig) | |||||||||
Method | X-RAY DIFFRACTION / Resolution: 2.3 Å | |||||||||
Authors | Kobe, B. / Deisenhofer, J. | |||||||||
Citation | Journal: J.Mol.Biol. / Year: 1996 Title: Mechanism of ribonuclease inhibition by ribonuclease inhibitor protein based on the crystal structure of its complex with ribonuclease A. Authors: Kobe, B. / Deisenhofer, J. #1: Journal: J.Mol.Biol. / Year: 1993 Title: Crystallization and Preliminary X-Ray Analysis of Porcine Ribonuclease Inhibitor, a Protein with Leucine-Rich Repeats Authors: Kobe, B. / Deisenhofer, J. #2: Journal: Nature / Year: 1993 Title: Crystal Structure of Porcine Ribonuclease Inhibitor, a Protein with Leucine-Rich Repeats Authors: Kobe, B. / Deisenhofer, J. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2bnh.cif.gz | 96.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2bnh.ent.gz | 73.2 KB | Display | PDB format |
PDBx/mmJSON format | 2bnh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bn/2bnh ftp://data.pdbj.org/pub/pdb/validation_reports/bn/2bnh | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 49092.156 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / Organ: LIVER / References: UniProt: P10775 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 3.87 Å3/Da / Density % sol: 68.24 % | ||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 21 ℃ / pH: 7 / Method: unknown | ||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: XUONG-HAMLIN MULTIWIRE / Detector: AREA DETECTOR / Date: 1992 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 24468 / % possible obs: 73.2 % / Observed criterion σ(I): 1 / Redundancy: 5.7 % / Rmerge(I) obs: 0.071 |
-Processing
Software |
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Refinement | Resolution: 2.3→40 Å / σ(F): 1 /
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Displacement parameters | Biso mean: 30.4 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.32 Å / Luzzati sigma a obs: 0.49 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.3→40 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |