+Open data
-Basic information
Entry | Database: PDB / ID: 2a0p | ||||||||||||||||||
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Title | Crystal structure of RNA oligomer containing 4'-thioribose | ||||||||||||||||||
Components | 5'-R(*Keywords | RNA / A-RNA / DOUBLE HELIX / RIBONUCLEIC ACID / 4'-THIO RNA | Function / homology | RNA | Function and homology information Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.95 Å | Authors | Haeberli, P. / Berger, I. / Pallan, P.S. / Egli, M. | Citation | Journal: Nucleic Acids Res. / Year: 2005 | Title: Syntheses of 4'-thioribonucleosides and thermodynamic stability and crystal structure of RNA oligomers with incorporated 4'-thiocytosine Authors: Haeberli, P. / Berger, I. / Pallan, P.S. / Egli, M. History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2a0p.cif.gz | 21.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2a0p.ent.gz | 14.8 KB | Display | PDB format |
PDBx/mmJSON format | 2a0p.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a0/2a0p ftp://data.pdbj.org/pub/pdb/validation_reports/a0/2a0p | HTTPS FTP |
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-Related structure data
Related structure data | 259dS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: RNA chain | Mass: 2572.658 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: synthesis route see publication #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.15 Å3/Da / Density % sol: 42.79 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: Cond 39 Hampton Screen I, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 128 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU / Wavelength: 1.5418 Å |
Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE / Date: 1997 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Highest resolution: 1.95 Å / Num. obs: 3077 / Observed criterion σ(I): 2 / Rmerge(I) obs: 0.067 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 259D Resolution: 1.95→25 Å / σ(F): 1 /
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Refinement step | Cycle: LAST / Resolution: 1.95→25 Å
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Refine LS restraints |
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