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Yorodumi- PDB-1zri: NOE-based solution structure with dipolar coupling restraints of ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1zri | ||||||
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Title | NOE-based solution structure with dipolar coupling restraints of rat OMP (olfactory marker protein) | ||||||
Components | Olfactory marker protein | ||||||
Keywords | SIGNALING PROTEIN / Beta-clamshell / omega-loop / Bex-binding protein | ||||||
Function / homology | Function and homology information neurogenesis / peptide binding / sensory perception of smell / axon / neuronal cell body / signal transduction / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Rattus norvegicus (Norway rat) | ||||||
Method | SOLUTION NMR / distance geometry simulated annealing | ||||||
Authors | Wright, N.T. / Margolis, J.W. / Margolis, F.M. / Weber, D.J. | ||||||
Citation | Journal: J.BIOMOL.NMR / Year: 2005 Title: LRefinement of the Solution Structure of Rat Olfactory Marker Protein (OMP) Authors: Wright, N.T. / Margolis, J.W. / Margolis, F.M. / Weber, D.J. | ||||||
History |
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Remark 650 | HELIX Author defined | ||||||
Remark 700 | SHEET Author defined |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1zri.cif.gz | 1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb1zri.ent.gz | 870.9 KB | Display | PDB format |
PDBx/mmJSON format | 1zri.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zr/1zri ftp://data.pdbj.org/pub/pdb/validation_reports/zr/1zri | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 18875.521 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: Omp / Plasmid: pGEX-KN / Production host: Escherichia coli (E. coli) / Strain (production host): JM109 / References: UniProt: P08523 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 3D and 4D homonuclear techniques, along with residual dipolar coupling experiments (IPAP-HSQC) |
-Sample preparation
Details |
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Sample conditions |
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-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | ||||||||||||||||||||
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Radiation wavelength | Relative weight: 1 | ||||||||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: distance geometry simulated annealing / Software ordinal: 1 Details: The structure is based on a total of 1764 restraints, of which 1343 are NOE-derived, 124 are H-bonds, 217 are dihedral angles, and 80 are residual dipolar coupling values | ||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |