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- PDB-1zo0: NMR structure of antizyme isoform 1 from rat -

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Basic information

Entry
Database: PDB / ID: 1zo0
TitleNMR structure of antizyme isoform 1 from rat
ComponentsOrnithine decarboxylase antizyme
KeywordsLYASE INHIBITOR / Antizyme / ornithine decarboxylase inhibitor
Function / homology
Function and homology information


negative regulation of polyamine transmembrane transport / Regulation of ornithine decarboxylase (ODC) / polyamine biosynthetic process / positive regulation of intracellular protein transport / ornithine decarboxylase inhibitor activity / positive regulation of protein catabolic process / protein-containing complex binding / enzyme binding / nucleus / cytosol / cytoplasm
Similarity search - Function
Ornithine decarboxylase antizyme 1 / Aminopeptidase - #60 / Ornithine decarboxylase antizyme / Ornithine decarboxylase antizyme superfamily / Ornithine decarboxylase antizyme / Ornithine decarboxylase antizyme signature. / Acyl-CoA N-acyltransferase / Aminopeptidase / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
Ornithine decarboxylase antizyme 1
Similarity search - Component
Biological speciesRattus norvegicus (Norway rat)
MethodSOLUTION NMR / simulated annealing
AuthorsHoffman, D.W. / Hackert, M.L.
CitationJournal: To be Published
Title: NMR structure of antizyme isoform 1 from rat
Authors: Hoffman, D.W. / Hackert, M.L.
History
DepositionMay 12, 2005Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jul 19, 2005Provider: repository / Type: Initial release
Revision 1.1Apr 30, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Mar 2, 2022Group: Database references / Derived calculations
Category: database_2 / pdbx_struct_assembly / pdbx_struct_oper_list
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.4May 22, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Ornithine decarboxylase antizyme


Theoretical massNumber of molelcules
Total (without water)14,2871
Polymers14,2871
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)12 / 12all calculated structures submitted
Representative

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Components

#1: Protein Ornithine decarboxylase antizyme / / ODC-Az


Mass: 14287.270 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Rattus norvegicus (Norway rat) / Production host: Escherichia coli (E. coli) / References: UniProt: P54370

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1112D NOESY
1213D 15N-separated NOESY
1313D 13C-separated ROESY

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Sample preparation

DetailsContents: 1 mM antizyme, 0.2 M sodium chloride, 20 mM phosphate buffer pH 7.0, 90% H2O, 10% D2O
Solvent system: 90% H2O/10% D2O
Sample conditionsIonic strength: 200 mM NaCl / pH: 7.0 / Pressure: ambient / Temperature: 295 K

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NMR measurement

RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M
Radiation wavelengthRelative weight: 1
NMR spectrometerType: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 500 MHz

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Processing

NMR softwareName: CNS / Version: 1.1 / Classification: refinement
RefinementMethod: simulated annealing / Software ordinal: 1
NMR ensembleConformer selection criteria: all calculated structures submitted
Conformers calculated total number: 12 / Conformers submitted total number: 12

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