SEQUENCE Residue 205 is a tyrosine in GB NP_227934 but no density for atoms beyond CB can be found ...SEQUENCE Residue 205 is a tyrosine in GB NP_227934 but no density for atoms beyond CB can be found in an otherwise ordered loop, suggesting sequencing error or spontaneous mutation. The authors suggest a point mutation either in the sequencing clone (SER->TYR mutation) or in their expression clone (TYR->SER). Codons for TYR=ATA and SER=AGA.
解像度: 2.4→19.98 Å / Cor.coef. Fo:Fc: 0.947 / Cor.coef. Fo:Fc free: 0.906 / SU B: 8.09 / SU ML: 0.192 / 交差検証法: THROUGHOUT / ESU R: 0.388 / ESU R Free: 0.261 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.24654
2969
5.1 %
RANDOM
Rwork
0.18444
-
-
-
obs
0.18767
54841
99.58 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK