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Open data
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Basic information
Entry | Database: PDB / ID: 1xj3 | ||||||
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Title | bjFixLH in unliganded ferrous form | ||||||
![]() | Sensor protein fixL![]() | ||||||
![]() | ![]() ![]() ![]() ![]() ![]() | ||||||
Function / homology | ![]() histidine phosphotransfer kinase activity / ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Key, J. / Moffat, K. | ||||||
![]() | ![]() Title: Crystal Structures of Deoxy and CO-Bound bjFixLH Reveal Details of Ligand Recognition and Signaling Authors: Key, J. / Moffat, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 39 KB | Display | ![]() |
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PDB format | ![]() | 26 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1xj2C ![]() 1xj4C ![]() 1xj6C ![]() 1drmS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 13101.703 Da / Num. of mol.: 1 / Fragment: heme domain Source method: isolated from a genetically manipulated source Details: reduced, unliganded form / Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() References: UniProt: P23222, ![]() |
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#2: Chemical | ChemComp-HEM / ![]() |
#3: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.57 Å3/Da / Density % sol: 59.3 % |
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Crystal grow![]() | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 9 Details: NaCl, PEI, CAPSO, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.9→40 Å / Num. all: 14771 / Num. obs: 13604 / % possible obs: 92.1 % / Observed criterion σ(I): -3 / Redundancy: 16.29 % / Rmerge(I) obs: 0.031 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 1DRM Resolution: 1.9→40 Å / Cross valid method: THROUGHOUT / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 1.9→40 Å
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Refine LS restraints |
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