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Yorodumi- PDB-1wsh: Crystal structure of E.coli RNase HI active site mutant (E48A/K87A) -
+Open data
-Basic information
Entry | Database: PDB / ID: 1wsh | ||||||
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Title | Crystal structure of E.coli RNase HI active site mutant (E48A/K87A) | ||||||
Components | Ribonuclease HI | ||||||
Keywords | HYDROLASE / RNase H / active-site mutant | ||||||
Function / homology | Function and homology information DNA replication, removal of RNA primer / ribonuclease H / RNA-DNA hybrid ribonuclease activity / endonuclease activity / nucleic acid binding / magnesium ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.9 Å | ||||||
Authors | Tsunaka, Y. / Takano, K. / Matsumura, H. / Yamagata, Y. / Kanaya, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1wsh.cif.gz | 131 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1wsh.ent.gz | 104.6 KB | Display | PDB format |
PDBx/mmJSON format | 1wsh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ws/1wsh ftp://data.pdbj.org/pub/pdb/validation_reports/ws/1wsh | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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4 |
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Unit cell |
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-Components
#1: Protein | Mass: 17506.859 Da / Num. of mol.: 4 / Mutation: E48A/K87A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Plasmid: pJAL600 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7Y4, ribonuclease H #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.24 Å3/Da / Density % sol: 45.05 % |
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-Data collection
Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL44XU / Wavelength: 0.9 Å |
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Detector | Type: MACSCIENCE / Detector: IMAGE PLATE / Date: Jun 21, 2003 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→50 Å / Num. all: 50326 / Num. obs: 50229 / % possible obs: 99.8 % |
Reflection shell | Highest resolution: 1.9 Å |
-Processing
Software | Name: CNS / Classification: refinement | |||||||||||||||
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.9→50 Å
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Refinement step | Cycle: LAST / Resolution: 1.9→50 Å
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