+Open data
-Basic information
Entry | Database: PDB / ID: 1wnk | ||||||
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Title | NMR Structure of FMBP-1 Tandem repeat 3 in 30%(V/V) TFE solution | ||||||
Components | Fibroin-Modulator-binding-protein-1 | ||||||
Keywords | TRANSCRIPTION / TANDEM REPEAT / N-CAP / DNA BINDING | ||||||
Function / homology | : / STPRs (score and three amino acid peptide repeats) / Fibroin-modulator-binding protein-1 Function and homology information | ||||||
Method | SOLUTION NMR / DISTANCE GEOMETRY, SIMULATED ANNEALING | ||||||
Authors | Yamaki, T. / Kawaguchi, K. / Aizawa, T. / Takiya, S. / Demura, M. / Nitta, K. | ||||||
Citation | Journal: To be Published Title: NMR Structure of FMBP-1 Tandem repeat 3 in 30%(V/V) TFE Solution Authors: Yamaki, T. / Kawaguchi, K. / Aizawa, T. / Takiya, S. / Demura, M. / Nitta, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1wnk.cif.gz | 114.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1wnk.ent.gz | 72 KB | Display | PDB format |
PDBx/mmJSON format | 1wnk.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wn/1wnk ftp://data.pdbj.org/pub/pdb/validation_reports/wn/1wnk | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 2710.129 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: SYNTHETIC CONSTRUCT / References: UniProt: Q5FBS0*PLUS |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D homonuclear techniques. |
-Sample preparation
Details |
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Sample conditions | pH: 3.2 / Pressure: ambient / Temperature: 293 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: JEOL ALPHA / Manufacturer: JEOL / Model: ALPHA / Field strength: 600 MHz |
-Processing
NMR software | Name: CNS / Version: 1.1 / Classification: refinement |
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Refinement | Method: DISTANCE GEOMETRY, SIMULATED ANNEALING / Software ordinal: 1 Details: the structures are based on a total of 266 restraints, 232 are NOE-derived distance constraints, 14 dihedral angle restraints, 20 distance restraints from hydrogen bonds. |
NMR representative | Selection criteria: lowest energy |
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 15 |