+Open data
-Basic information
Entry | Database: PDB / ID: 1wi2 | ||||||
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Title | Solution structure of the PDZ domain from RIKEN cDNA 2700099C19 | ||||||
Components | RIKEN cDNA 2700099C19 | ||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information Biotin transport and metabolism / Transport of vitamins, nucleosides, and related molecules / Ion influx/efflux at host-pathogen interface / Vitamin B5 (pantothenate) metabolism / protein localization to basolateral plasma membrane / Ion transport by P-type ATPases / maintenance of epithelial cell apical/basal polarity / : / pore complex assembly / neurotransmitter secretion ...Biotin transport and metabolism / Transport of vitamins, nucleosides, and related molecules / Ion influx/efflux at host-pathogen interface / Vitamin B5 (pantothenate) metabolism / protein localization to basolateral plasma membrane / Ion transport by P-type ATPases / maintenance of epithelial cell apical/basal polarity / : / pore complex assembly / neurotransmitter secretion / pore complex / adherens junction / cell-cell junction / presynapse / basolateral plasma membrane / synapse / cytosol Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | SOLUTION NMR | ||||||
Authors | Seimiya, K. / Tomizawa, T. / Kigawa, T. / Yoshida, M. / Hayashi, F. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: to be published Title: Solution structure of the PDZ domain from RIKEN cDNA 2700099C19 Authors: Seimiya, K. / Tomizawa, T. / Kigawa, T. / Yoshida, M. / Hayashi, F. / Yokoyama, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1wi2.cif.gz | 567.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1wi2.ent.gz | 469 KB | Display | PDB format |
PDBx/mmJSON format | 1wi2.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wi/1wi2 ftp://data.pdbj.org/pub/pdb/validation_reports/wi/1wi2 | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11108.491 Da / Num. of mol.: 1 / Fragment: PDZ domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Description: Cell-free protein synthesis / Gene: RIKEN cDNA 2700099C19 / Plasmid: P030203-31 / References: UniProt: Q9CZG9 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 0.41mM 13C, 15N-labeled protein; 20mM phosphate buffer; 100mM NaCl; 1mM d-DTT; 0.02% NaN3 Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 120mM / pH: 6.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 800 MHz |
-Processing
NMR software |
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NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations, target function Conformers calculated total number: 100 / Conformers submitted total number: 20 |