+Open data
-Basic information
Entry | Database: PDB / ID: 1wac | ||||||
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Title | Back-priming mode of Phi6 RNA-dependent RNA polymerase | ||||||
Components | P2 PROTEIN | ||||||
Keywords | POLYMERASE / PHI6 RNA-DEPENDENT RNA POLYMERASE / TRANSCRIPTION | ||||||
Function / homology | Function and homology information RNA uridylyltransferase activity / virion component / RNA-directed RNA polymerase / viral RNA genome replication / RNA-dependent RNA polymerase activity / nucleotide binding / DNA-templated transcription / RNA binding / metal ion binding Similarity search - Function | ||||||
Biological species | PSEUDOMONAS PHAGE PHI-6 (bacteriophage) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3 Å | ||||||
Authors | Laurila, M.R.L. / Salgado, P.S. / Stuart, D.I. / Grimes, J.M. / Bamford, D.H. | ||||||
Citation | Journal: J.Gen.Virol. / Year: 2005 Title: Back-Priming Mode of Phi6 RNA-Dependent RNA Polymerase Authors: Laurila, M.R.L. / Salgado, P.S. / Stuart, D.I. / Grimes, J.M. / Bamford, D.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1wac.cif.gz | 383.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1wac.ent.gz | 316.4 KB | Display | PDB format |
PDBx/mmJSON format | 1wac.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wa/1wac ftp://data.pdbj.org/pub/pdb/validation_reports/wa/1wac | HTTPS FTP |
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-Related structure data
Related structure data | 1hi8S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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3 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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-Components
#1: Protein | Mass: 74440.648 Da / Num. of mol.: 3 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) PSEUDOMONAS PHAGE PHI-6 (bacteriophage) Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: P11124 Compound details | ENGINEERED RESIDUE MET 465 ILE, CHAINS A, B, C DELETION MUTATION- QYKW (629-632) TO SG IN CHAINS A, ...ENGINEERED | Sequence details | DELETION MUTATION- QYKW (629-632) TO SG IN CHAINS A, B AND C. THE TWO RESIDUES WHICH HAVE BEEN ...DELETION MUTATION- QYKW (629-632) TO SG IN CHAINS A, B AND C. THE TWO RESIDUES WHICH HAVE BEEN INSERTED IN PLACE OF QYKW ARE MAPPED TO THEMSELVES | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.83 Å3/Da / Density % sol: 50 % |
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Crystal grow | pH: 8 Details: 0.1M SODIUM CITRATE PH 5.6, 19% ISOPROPANOL, 19% PEG 4K, 5% GLYCEROL |
-Data collection
Diffraction | Mean temperature: 150 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID29 / Wavelength: 0.9756 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9756 Å / Relative weight: 1 |
Reflection | Resolution: 3→30 Å / Num. obs: 57385 / % possible obs: 99.2 % / Observed criterion σ(I): 1.9 / Redundancy: 10.8 % / Biso Wilson estimate: 23.9 Å2 / Rmerge(I) obs: 0.02 / Net I/σ(I): 7.4 |
Reflection shell | Resolution: 3→3.1 Å / Mean I/σ(I) obs: 1.9 / % possible all: 98.6 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1HI8 Resolution: 3→20 Å / Rfactor Rfree error: 0.005 / Data cutoff high absF: 1769196.42 / Isotropic thermal model: RESTRAINED / Cross valid method: A POSTERIORI / σ(F): 0 Details: RESIDUES 1-5, 603-615, 626-634 BELONG TO DISORDERED LOOPS
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Solvent computation | Solvent model: CNS BULK SOLVENT MODEL USED / Bsol: 45.2045 Å2 / ksol: 0.371779 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 35.65 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3→20 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: CONSTRAINTS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 3→3.19 Å / Rfactor Rfree error: 0.017 / Total num. of bins used: 6
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