+Open data
-Basic information
Entry | Database: PDB / ID: 1wab | ||||||
---|---|---|---|---|---|---|---|
Title | PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE | ||||||
Components | PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE1-alkyl-2-acetylglycerophosphocholine esterase | ||||||
Keywords | PLATELET FACTOR / OXIDOREDUCTASE / FLAVOPROTEIN / FAD / PEROXISOME | ||||||
Function / homology | Function and homology information platelet-activating factor acetyltransferase activity / 1-alkyl-2-acetylglycerophosphocholine esterase complex / COPI-independent Golgi-to-ER retrograde traffic / 1-alkyl-2-acetylglycerophosphocholine esterase / 1-alkyl-2-acetylglycerophosphocholine esterase activity / lipid catabolic process / spermatogenesis / protein heterodimerization activity / protein homodimerization activity / cytoplasm Similarity search - Function | ||||||
Biological species | Bos taurus (cattle) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 1.7 Å | ||||||
Authors | Ho, Y.S. / Swenson, L. / Derewenda, U. / Serre, L. / Wei, Y. / Dauter, Z. / Hattori, M. / Aoki, J. / Arai, H. / Adachi, T. ...Ho, Y.S. / Swenson, L. / Derewenda, U. / Serre, L. / Wei, Y. / Dauter, Z. / Hattori, M. / Aoki, J. / Arai, H. / Adachi, T. / Inoue, K. / Derewenda, Z.S. | ||||||
Citation | Journal: Nature / Year: 1997 Title: Brain acetylhydrolase that inactivates platelet-activating factor is a G-protein-like trimer. Authors: Ho, Y.S. / Swenson, L. / Derewenda, U. / Serre, L. / Wei, Y. / Dauter, Z. / Hattori, M. / Adachi, T. / Aoki, J. / Arai, H. / Inoue, K. / Derewenda, Z.S. #1: Journal: J.Biol.Chem. / Year: 1994 Title: The Catalytic Subunit of Bovine Brain Platelet-Activating Factor Acetylhydrolase is a Novel Type of Serine Esterase Authors: Hattori, M. / Adachi, H. / Tsujimoto, M. / Arai, H. / Inoue, K. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1wab.cif.gz | 60.5 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1wab.ent.gz | 44.4 KB | Display | PDB format |
PDBx/mmJSON format | 1wab.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wa/1wab ftp://data.pdbj.org/pub/pdb/validation_reports/wa/1wab | HTTPS FTP |
---|
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
| ||||||||
Components on special symmetry positions |
|
-Components
#1: Protein | Mass: 25903.363 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: WITH ACETATE BOUND / Source: (natural) Bos taurus (cattle) / Organ: BRAIN References: UniProt: Q29460, 1-alkyl-2-acetylglycerophosphocholine esterase |
---|---|
#2: Chemical | ChemComp-ACT / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
---|
-Sample preparation
Crystal | Density Matthews: 2.67 Å3/Da / Density % sol: 48.5 % | ||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | *PLUS pH: 7.5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction source | Source: SYNCHROTRON / Site: EMBL/DESY, HAMBURG / Beamline: BW7B / Wavelength: 0.859 |
---|---|
Detector | Type: MARRESEARCH / Date: Nov 25, 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.859 Å / Relative weight: 1 |
Reflection | Num. obs: 33716 / % possible obs: 99.7 % / Redundancy: 11.5 % / Rmerge(I) obs: 0.063 |
Reflection | *PLUS Highest resolution: 1.65 Å / Lowest resolution: 20 Å / Num. measured all: 387544 |
-Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 1.7→8.5 Å /
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 23.1 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.7→8.5 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor all: 0.1954 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 23.108 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: p_angle_d / Dev ideal: 0.034 |