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Yorodumi- PDB-1uhp: Solution structure of RSGI RUH-005, a PDZ domain in human cDNA, K... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1uhp | ||||||
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Title | Solution structure of RSGI RUH-005, a PDZ domain in human cDNA, KIAA1095 | ||||||
Components | hypothetical protein KIAA1095Hypothesis | ||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / PDZ domain / Semaphorin cytoplasmic domain associated protein / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information neuromuscular junction development / RING-type E3 ubiquitin transferase / neuromuscular junction / ubiquitin-protein transferase activity / ubiquitin protein ligase activity / protein ubiquitination / zinc ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Hamada, T. / Muto, Y. / Hirota, H. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: To be published Title: Solution structure of RSGI RUH-005, a PDZ domain in human cDNA, KIAA1095 Authors: Hamada, T. / Muto, Y. / Hirota, H. / Yokoyama, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1uhp.cif.gz | 605.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1uhp.ent.gz | 503 KB | Display | PDB format |
PDBx/mmJSON format | 1uhp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/uh/1uhp ftp://data.pdbj.org/pub/pdb/validation_reports/uh/1uhp | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11081.239 Da / Num. of mol.: 1 / Fragment: PDZ domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Description: Cell-free protein synthesis / Gene: KIAA1095 / Plasmid: P021030-36 / References: UniProt: Q9UPQ7 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using 3D NMR techniques |
-Sample preparation
Details | Contents: 1.1mM PDZ domain U-15N,13C; 20mM Tris-HCl buffer (pH 7.0); 100mM NaCl; 0.02% NaN3; 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 100mM NaCl / pH: 7.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 800 MHz |
-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations, structures with the lowest energy, target function Conformers calculated total number: 100 / Conformers submitted total number: 20 |