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- PDB-1u73: Crystal structure of a Dimeric Acidic Platelet Aggregation Inhibi... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1u73 | ||||||
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Title | Crystal structure of a Dimeric Acidic Platelet Aggregation Inhibitor and Hypotensive Phospholipase A2 from Bothrops jararacussu | ||||||
![]() | hypotensive phospholipase A2 | ||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Magro, A.J. / Murakami, M.T. / Soares, A.M. / Arni, R.K. / Fontes, M.R. | ||||||
![]() | ![]() Title: Crystal structure of an acidic platelet aggregation inhibitor and hypotensive phospholipase A(2) in the monomeric and dimeric states: insights into its oligomeric state Authors: Magro, A.J. / Murakami, M.T. / Marcussi, S. / Soares, A.M. / Arni, R.K. / Fontes, M.R. #1: ![]() Title: Structural and Functional Characterization of an Acidic Platelet Aggregation Inhibitor and Hypotensive Phospholipase A2 from Bothrops jararacussu Venom Authors: Andriao-Escarso, S.H. / Soares, A.M. / Fontes, M.R. / Fuly, A.L. / Correa, F.M. / Rosa, J.C. / Greene, L.J. / Giglio, J.R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 66.4 KB | Display | ![]() |
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PDB format | ![]() | 49.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 13700.451 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Details: Venom glands / Source: (natural) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.733 Å3/Da / Density % sol: 29.04 % |
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Crystal grow![]() | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 3.5 Details: ammonium sulfate, PEG 6000, pH 3.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Feb 15, 2002 / Details: Mirrors |
Radiation | Monochromator: MIRRORS / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.9→29.7 Å / Num. all: 15160 / Num. obs: 14151 / % possible obs: 93.3 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 3.5 % / Biso Wilson estimate: 24.8 Å2 / Rmerge(I) obs: 0.045 / Net I/σ(I): 20.1 |
Reflection shell | Resolution: 1.9→2.02 Å / Redundancy: 3.4 % / Rmerge(I) obs: 0.293 / Mean I/σ(I) obs: 5 / Num. unique all: 2084 / % possible all: 87.7 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: BthA-I (monomeric form) Resolution: 1.9→29.7 Å / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 27.8 Å2
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Refine analyze | Luzzati coordinate error obs: 0.2 Å / Luzzati d res low obs: 4 Å / Luzzati sigma a obs: 0.22 Å | |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→29.7 Å
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Refine LS restraints |
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